IHC
Buffers
Phosphate Buffered Saline 0.1M, pH 7.4
(use: Rinsing unfixed tissues and diluting antibodies, eg. in IHC.)
Store at 2-8°C.
Tris Buffered Saline 0.05M, pH 7.6, 10X
(use: IHC wash solution; dilute 1:10 before use.)
Store at 2-8°C.
Retrieval Solutions
Uni-Trieve Solution
This product is manufacturered by Innovex Biosciences.
Click Here for part number, pricing and a tutorial on how it works.
Background Eradicators
Fc Receptor Blocker
Eliminates non-specific staining caused by Fc receptor staining in lymphoid and hematopoietic cells.
Fc Receptor Blocker, Azide Free
Preservative-free, Antibody-free Fc Blocker for human, mouse and all animal cells.
Innovative Support Reagents & Buffers
Enhancing Wash Buffers
This product is located in the IHC/Chromogens Section.
Click Here for part number and pricing.
Immuno Diluent and Block
This product is located in the IHC/Innovex Biosciences Section.
Click Here for part number and pricing.
Innovex Cyto-Blue (Permanent)
Blue cytoplasmic stain developed especially for counterstaining IHC nuclear stains of ER, PR, P53, PCNA, etc. Does not bind to nucleus. No masking of AEC or DAB stained nuclei occurs. Mountable with Innovex Probe Mount or xylene based mounting media.
Innovex Nuclear Red
Red color counterstain for IHC-BCIP/NBT stains, in-situ probes, and special stains. Mountable with xylene based media or Innovex Probe Mount. No fading with time.
Isotonic Buffered Saline (PBS)
An all purpose PBS for laboratory use. No preservatives. 20X concentrate, Store at 15-30°C. Shelf life 3 years.
STAT Aqua Hematoxylin (Permanent)
Allows for Stat nuclear counter and special staining in 10 seconds. Specially developed for IHC stains. Mountable with xylene and aqueous based mounting media. Stain does not fade with time, no precipitation,no filtration. Usable to the last drop. Shelf life 4 years. Store at 4°C.
Tissue Recovery Solution with Built-in pH Stabilizer
Innovex’s unique non-citrate formulation with built-in pH stabilizer provides optimal and gentle unmasking of antigens in the various heat methods (including high temp). Optimal pH remains constant regardless of temperature. Non-damaging to tissue morphology. Tissue does not slough off the slide. Shelf life 3 years. Store at 4°C.
Mounting Media
ADVANTAGE Permanent Mounting Media
Permanently preserves AEC, DAB, Fast Red and other immuno stains plus dye chemistry stains. Sets in 5 minutes at RT.
This product is located in the Slides & Cover Slips Section.
Click Here for part number and pricing.
H&E Mount
Unique, permanent mounting media designed for coverslipping H&E stains from water or alcohol. Reduce time in eosin if cover slipping from water. Sets in 10 minutes.
This product is located in the Slides & Cover Slips Section.
Click Here for part number and pricing.
Probe Mount
Nuclear Fast Red stained specimens can be permanently mounted. Mount immunofluorescence & in-situ slides from water. Sets in 5 minutes.
This product is located in the Slides & Cover Slips Section.
Click Here for part number and pricing.
Antigen Retrieval
Uni-Trieve
Uni-Trieve User Friendly Protocol:
Uni-Trieve is a mild-temperature universal retrieval solution that retrieves all antibodies, on all human and all animal tissues at only 65°C-70°C for 30 minutes using a simple water bath.
- Is Totally Background-Free and unlike citrate buffer, High pH buffers, pressure cookers or microwaves, no background or tissue damage or loss is observed
- Standardizes retrieval, it retrieves all tissues with a single solution.
- Gentle temperature for immunofluorescence (IF), delicate tissues such as brain, bone marrow and animal tissues.
- OVER-NIGHT Retrieval is possible: simply immerse sides in 40°C-45°C Uni-Trieve using a water bath as the heat source. Next morning remove slides from the water bath, rinse and proceed with IHC or IF staining!
Chromogens
Alkaline Phosphatase-Enhancing Wash Buffer
(For amplifying Fast Red, BCIP/NBT, Innovex Brown & other substrate chromogens for alkaline phosphatase enzyme staining.)
