Pigment, Substance & Mineral Stains
- Shelf Life for most Stain Kits is one year from date manufactured.
- Store Stain Kits at 15-30°C unless otherwise stated.
- Most Stain Kits include 2 Complimentary Positive Control Slides to be used for the initial verification of staining techniques and reagents.
- Stain solution groups are sold as individual products under separate part numbers with storage requirements and expiration date designated per bottle.
|Copper||Some methods demonstrate copper associated protein rather than copper itself||
Copper Stain Kit
|Hemosiderin||Prussian Blue reaction stains ferric (+3) ions||
Iron Stain Kit
|Hematoidin||Similar to bilirubin often formed as a result of hemorrhage, stains with bile methods but not with iron methods|
|Bile||Demonstrated when bilirubin is oxidized to biliverdin in an acid staining environment||
|Calcium||Black deposits formed when using silver reactions are due to reduction of silver by organic material followed by exposure to strong light||
Both forms are water soluble, aqueous fixatives should be avoided
Absolute ethanol is the fixative of choice
Gout – monosodium urate crystals appear yellow when their long axes are aligned parallel to a red compensator filter
Pseudogout -calcium pyrophosphate crystals, appear blue when their long axes are aligned parallel to a red compensator filter
|Melanin||Identified by a number of methods can interfere with pathology interpretation if found in large amounts||
|Lipofusin||Stains variably but usually PAS positive||PAS Stain Kit||9162|
|Carbon||Inert and unreactive, resists removal procedures, commonly found in lung and mediastinal lymph nodes.|
|Melanin||Can become problematic in large amounts and be removed by a variety of methods||Tech Memo|
|Formalin||Formed in unbuffered formalin when pH shifts to acidic, not reactive with iron staining methods||Tech Memo|
|Mercury||Deposited as a result of using mercury based fixatives||Tech Memo|