Fc Receptor Blocker

Innovex Fc Receptor Blocker is a peptide based technology designed to block Fc Receptors present on all leukocytes (white blood cells), lymphomas, leukemias and melanomas.  Fc receptors are also expressed on a majority of tumors. Blocking the Fc receptors is essential for accurate typing of lymphoid tumors.

APPLICATION/INTENDED USE OF FC RECEPTOR BLOCKER:

This reagent can be universally used to block all types of Fc receptors for all species’ cells including human, mouse and all animal species cells and tissues by a variety of immunoassays such as IHC, Immunofluorescence (IF) and Flow cytometry.

FC RECEPTOR BLOCKER FEATURES AND BENEFITS:

• Blocks Fc Receptors for both human and animal cells/tissues
• 30 minute incubation step at room temperature
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow), melanoma tumors and other Fc expressing tumors
• Ensures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells

STORAGE CONDITIONS:

Store in refrigerator at 2-8°C through the expiration date noted on the vial label.

PRODUCT FORMAT:

Working solution, no dilution or adjustments required.

INSTRUCTIONS FOR USE:

For Blocking Fc Receptors in Immunohistochemical and Immunofluorescence Specimens:

1. Deparaffinize paraffin section slides or cut frozen sections, fix and rinse in water as usual.
2. Quench endogenous peroxidase by immersion in 3% H₂O₂ (Only for Peroxidase-IHC staining).
3. Cover sections or smears with 3-6 drops of Fc receptor block to achieve full specimen coverage.
4. Incubate for 30 minutes at room temperature.
5. Rinse with rinse buffer.
6. Proceed with the remainder of IHC staining steps per lab protocol.

Innovex Fc Receptor Blocker must be used prior to use of any other blocker, e.g. serum or protein blocks.

Innovex Fc Receptor Blocker can be used in autostainers as a pre-treatment step prior to application of protein and/or serum blocking.

For Immunofluorescence labeling of tissues and cell preparations:

Deparaffinize paraffin section slides OR cut frozen sections, fix and rinse in water as usual.

1. Cover tissue sections or cell preps with 3-6 drops of Fc Receptor Blocker to achieve full specimen coverage.
2. Incubate for 30 minutes at room temperature.
3. Rinse with PBS or DI water and proceed with application of fluorochrome-conjugated antibody (direct method) OR with the application of non-conjugated primary antibody followed by fluorochrome conjugated secondary antibody (indirect method)

Innovex Fc Receptor Blocker must be used prior to use of any other blocker, e.g. serum or protein blocks or IF Blockers.

For Flow Cytometry Blocking of Fc Receptors:

1. Lyse or ficol blood as usual OR use whole blood.
2. Add 150-300 micro liter of Fc Receptor Blocker per 1,000,000 cells.
3. Incubate for 30 minutes on ice OR at room temperature.
4. Wash twice in assay wash buffer.
5. Proceed with antibody labeling.