Advantage
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- Permanent Mounting medium
- Preserves AEC, DAB, Fast Red and other dye chemistry stains
- Sets in 5 minutes at Room Temp.
- Manufactured by Innovex Biosciences
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ADVANTAGE™ Mounting Media is formulated for permanently mounting (coverslipping) slides without the need for xylene and alcohol pre-treatment steps.
FEATURES OF ADVANTAGE™ MOUNTING MEDIA:
- Mount permanently and directly from water.
- Permanently preserves AEC, DAB, Fast Red & other immuno stains.
- No fading of chromogens or dyes.
- Fluid is at room temperature, no warming is necessary.
- No fume hood needed to mount.
- Sets in 5 minutes at room temperature, ready for microscopic examination.
- Permanently preserves IHC stains and special stains.
- Saves time, mounting time of less than 10 seconds per slide.
ADVANTAGE™ MOUNTING MEDIA APPLICATION:
Advantage™ is intended for the coverslipping stained biological specimen. It is intended to be applied with coverslips. It can also be used for making wet mounts.
ADVANTAGE™ MOUNTING MEDIA, MOUNTING & DRYING PROTOCOL:
The following is a recommended technique for coverslipping slide specimens. Other techniques that achieve the same basic result are equally acceptable.
- Take the slides from the final wash (for best results water is quite suitable).
- Remove excess water by air drying, maximum removal of water is recommended.
- Place the slides down, face up on a flat surface and apply 2-3 drops (or as needed) of Advantage mounting media on the lower edge of the coverslip.
- Bring the slide up to the edge of the coverslip and invert the slide so that the mounting media touches the slide. Gently complete inversion.
- For immediate viewing, Quick-Set the coverslip by placing the mounting slides in an oven for the following time: 5 minutes at 37º C or let the slides sit at room temperature for 10-15 minutes. IMPORTANT: Do not leave slides at 37º for more than one hour. Slides left longer than one hour at oven temperature may exhibit small bubble formations.
- Mounted slides should be properly stored following the microscopic examination. Slides are best stored in the dark and free of dust.
SECOND CHANCE COVERSLIPPING:
If for any reason you end up with an unsatisfactory mounted slide such as one with some air bubbles, remount slides as follows:
- Allow the coverslip to slip off the end of the slide by holding the slide at a slight vertical angle.
- Remove excess media as if it were excess water on a slide.
- Re-apply mounting media and coverslip.
- Mounted slides should be properly stored following the microscopic examination. Slides are best stored in the dark and free of dust.
COVERSLIP REMOVAL:
When desired, coverslips mounted with Advantage™ Mounting Media may be removed by soaking the slides in warm water. The length depends on the age of the mounted slide and may vary from 10 minutes to over-night. After softening the mounting media, slowly and gently pull back on the corner of the coverslip until it releases. Then rinse off the remaining mounting media by agitating the un-slipped slide in the warm water for a few moments.