Decalcifying Solutions
- Shelf Life is one year from date manufactured unless otherwise stated.
- Store at 15-30°C unless otherwise stated.
- H = Hazardous
| Decalcifying Solution | Rate | Morphology | Recommend Use | Formulation | Buffer | Surface Decal | Shelf Life |
| 1050 HCL/EDTA |
Rapid | Good | All specimen types. Rapid acid decalcification with EDTA to help protect tissue/cell morphology. | 10% HCL EDTA Tetrasodium | Tartrate Buffered | Not recommended | 2 Yrs |
| 1048 EDTA/Sucrose | Slow | Excellent | Preservation of tissue morphology, antigenicity & nucleic acid integrity. | 20% EDTA 5% Sucrose | Tris Buffered | Not recommended | 1 Yr |
| 1049 Formic Acid 5%, Aqueous |
Moderate | Very Good | General purpose decalcification. | 5% Formic Acid | Unbuffered | Yes | 1 Yr |
| 10492 Formic Citrate |
Moderate | Excellent | Suitable for all specimen types, well suited for compact bone. | 25% Formic Acid | Citrate Buffered | Yes | 2 Yrs |
| 10493 Formic Acid/Formalin |
Moderate | Good to Very Good | 1-step fixation & decalcification of light bone specimens. Tissue fixation prior to use improves morphology. | 5% Formic Acid 5% Formaldehyde |
Unbuffered | Not recommended | 2 Yrs |
| 10491 Formic/Formate |
Moderate | Excellent | Suitable for all specimen types, well-suited for compact bone. | 9% Formic Acid | Formate Buffered | Not recommended | 2 Yrs |
Decalcification End-Point Set #1051: Used to determine complete decalcification if radiography is not available.
Decalcifying Solution, HCL/EDTA
(use: Excellent for bone marrows. Gentle but quick!)
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- Shelf Life is 2 years from date of manufacture.
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Decalcification End Point Set
(use: Determination of complete decalcification of specimen.)
Solution A: Ammonium Hydroxide, Aqueous 250 ml
Solution B: Ammonium Oxalate, Aqueous 250 ml

