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Fc Receptor Blocker, Azide Free

Fc Receptor Blocker by Innovex

Fc Receptor Blocker, Azide Free does not contain sodium azide or thimerosol and it is therefore suitable for effective blocking of Fc receptors in non-fixed live cells of lymphoid and hematopoietic origin as well as tumor cells and cell lines that carry Fc receptors on their surface.

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Part # NB335 30 ml $490.00

Fc Receptor Blocker, Azide Free can be used as a blocking agent to block Fc receptors present on hematopoetic (blood derived), lymphoid cells/tissues and tumor cells and cell lines.  The binding of the Fc region of the primary or the secondary antibody to the Fc receptors present on the surface of leukocytes (white blood cells) or tumor cells gives rise to non-specific binding and is observed as background or non-specific staining in IHC ICC, immunofluorescence and flow cytometry assays.

 

Fc Receptor Blocker, Azide Free does not contain sodium azide or thimerosol and it is therefore suitable for effective blocking of Fc receptors in non-fixed live cells of lymphoid and hematopoietic origin as well as tumor cells and cell lines that carry Fc receptors on their surface.  Fc Receptor Blocker, Azide Free is especially helpful for blocking Fc receptors in tumor cells or cell lines employed in functional assays where sodium azide and other preservatives cause endocytosis and result in cytotoxicity and cell damage.

 

STORAGE CONDITIONS:

Store in refrigerator at 2-8°C through the expiration date noted on the vial label.  DO NOT FREEZE.

 

PRODUCT FORMAT:

Working solution, NO dilution or adjustments necessary.

 

INSTRUCTIONS FOR USE OF THE FC RECEPTOR BLOCKER AZIDE FREE:

For blocking Fc receptors in live cells and for use in functional assays:

Fc Receptor Blocker Azide Free is used for blocking Fc receptors in live cells and cell lines assays that employ live and non-fixed cells; and further for functional assays where presence of sodium azide and other preservatives cause endocytosis, cytotoxicity and cell damage.  Live cell assays and functional assays are varied and are many in numbers.

 

To block Fc receptors, present on live cells and cell lines that express Fc receptors:

Live cells must be exposed to Innovex Fc Blocker for a period of at least 30 minutes.  The optimal incubation time with Fc Blocker must be determined by the lab user for the specific cells, cell lines, species and the assay condition employed.

  1. Add 0.2 to 0.5 ml of Fc Receptor Blocker Azide Free for 106 (1 million) cells.
  2. Incubate for 30 minutes to 1 hour on ice or at room temperature.
  3. Rinse with assay wash buffer or DI water.
  4. Proceed with assay.

 

For Flow Cytometry:

  1. Use whole blood OR lyse or ficol blood as usual.
  2. Add 0.2 ml of Fc Receptor Blocker Azide Free for 106 (1 million) cells.
  3. Incubate for 30 minutes on ice or at room temperature.
  4. Wash twice in assay wash buffer.
  5. Proceed with antibody labeling.