Products

PAP Pen Liquid Blocker

PAP PEN LIQUID BLOCKER Super Pap Pen by Daido Sangyo

This newest generation of Pap Pens is still a superior hydrophobic liquid, which creates a secure boundary on glass and plastic around a tissue specimen during IHC procedures. Redesigned in 2001 for in-situ methods and stronger adherence. Fluid flows as a light blue on the slide and is easier to see than original (clear) pap pens.

PAP PEN LIQUID BLOCKER Super Pap Pen Tip by Daido Sangyo

Click here for  information on the Mini Pap Pen Liquid Blocker

Product Options:

Part # 6505 each $85.00
Part # 6505 6 or more $80.90

PRODUCTS:                                                       

  Each or 6/Set
PAP Pen Liquid Blocker Part 6505
PAP Pen Liquid Blocker, Mini Part 6506

 

APPLICATION:

Newcomer Supply PAP Pen Liquid Blocker, hydrophobic slide markers creates a visible hydrophobic barrier that provides proper surface tension to hold reagents within a targeted area when applied around tissue sections or smears on a microscopic slide. This barrier ensures the amount of reagent needed is reduced and retained for complete tissue coverage and staining reaction. Multiple specimens can be separated by drawn circles or lines applied to the same slide. The PAP Pen, Mini provides a finer pen tip for drawing a thinner barrier film.

PAP Pen Liquid Blockers contain a unique formulation that is water repellent, insoluble in alcohol and acetone, soluble in xylene and temperature resistant up to 120°C.

 

METHOD:

Technique:  Paraffin, frozen sections and smears

      • Manual immunohistochemistry (IHC) procedures
      • Manual immunofluorescence (IF) procedures
      • Manual In-Situ hybridization (ISH) procedures
      • Manual enzyme procedures

 

PAP PEN ACTIVATION INSTRUCTIONS:

    1. Vigorously shake capped pen for approximately 10 seconds.
    2. Remove cap; hold pen upright and depress tip 2-3 times.
    3. Invert pen, press tip lightly on absorbent paper until tip is visually saturated. Release pressure on tip and blot away any excess liquid.
    4. Practice applying a thin PAP Pen liquid barrier on a test slide using minimal pressure. Too much pressure may result in excess barrier solution creating a wider film that could touch tissue edges.
    5. Store PAP Pen Liquid Blocker at room temperature, tightly capped on a horizontal flat surface.
    6. Pen re-activation not required unless tip dries out during storage.

 

PROCEDURE:

    1. Paraffin Section Method:
        1. Deparaffinize sections thoroughly in xylene and hydrate through 100% and 95% ethyl alcohols. Wash well with distilled water.
        2. Remove slide from water, blot excess solution from slide and tissue section. Or place long edge of slide on absorbent material to remove excess moisture.
        3. Encircle tissue section on slide surface with PAP Pen as illustrated. Do not touch the pen to any edges of the tissue.
        4. The liquid barrier can be drawn on a slightly damp slide.
        5. See Procedure Notes #1 and #2.
    2. Frozen Section and Smear Method:
        1. Encircle frozen tissue section or smear on slide at room temperature with the PAP Pen. Do not touch the pen to any edges of the tissue section or smear.
        2. The PAP Pen barrier should be applied before fixation or prior to immersion of slide into water or buffer.
        3. See Procedure Notes #1 and #2.
    3. After liquid barrier application, allow barrier film to dry before proceeding with staining procedure.
        1. See Procedure Note #3.
    1. Drain/rinse reagents off between staining steps; blotting slide and tissue as needed to remove excess solution.
        1. See Procedure Note #4.
    2. Complete staining; coverslip with compatible mounting medium.

 

PROCEDURE NOTES:

    1. Once a tissue section is touched with PAP Pen liquid barrier it cannot be removed. The section remains usable but colorization will result on the touched portion of tissue.
    2. If the tissue section is not completely encircled by the PAP Pen Liquid Blocker barrier film, reagents will not be fully retained on the section and flood out onto the slide. This may compromise adequate tissue coverage by reagents.
    3. If liquid barrier lines are not completely dry prior to staining, a precipitate from reaction with reagents may occur.
    4. The use of Slide Moisture Chamber (68431, 68432) or StainTray™ (6847, 6848) is recommended for manual staining to maintain slide organization and a moist environment during the procedure.

 

REFERENCES:

    1. Grizzle, William, Cecil Stockard and Paul Billings. “The Effects of Tissue Processing Variables Other Than Fixation on Histochemical Staining and Immunohistochemical Detection of Antigens.” The Journal of Histotechnology 24.3 (2001): 213-219.
    2. Vidwans, Malavika, Srinivas Mandavilli, Wanda Nethers and Richard Cartun. “Fine-Needle Aspiration Diagnosis of a Neck Mass Using Immunocytochemical Stains Performed on Stained Cytology Slides.” The Journal of Histotechnology 25.4 (2002): 275-277.
    3. Modifications developed by Newcomer Supply Laboratory.