PAP GnomePen Classic Liquid Blocker
PAP GnomePen liquid blocker is a proprietary formulation that is applied around the tissue sections on a microscope slide prior to immunohistochemistry. It dries in less than one minute!
PRODUCTS:
Each | |
PAP GnomePen™ Classic – Blue | Part 6507B |
PAP GnomePen™ Classic – Red | Part 6507R |
PAP GnomePen™ Classic – Green | Part 6507G |
PAP GnomePen™ Classic – Orange | Part 6507E |
PAP GnomePen™ Classic – Violet | Part 6507U |
PAP GnomePen™ Classic – Yellow | Part 6507Y |
PAP GnomePen™ Flat – Blue | Part 6508B |
PAP GnomePen™ Flat – Red | Part 6508R |
PAP GnomePen™ Flat – Green | Part 6508G |
PAP GnomePen™ Flat – Orange | Part 6508E |
PAP GnomePen™ Flat – Violet | Part 6508U |
PAP GnomePen™ Flat – Yellow | Part 6508Y |
APPLICATION:
Newcomer Supply PAP GnomePen™ Liquid Blocker available in both “classic” thin tip and “flat” wider tip, creates a visible hydrophobic film around tissue sections to reduce reagent volume needed for tissue coverage and stain reaction. Both classic and flat PAP GnomePen™are available in multiple barrier colors for color coding and specimen differentiation. Each pen contains 250-350 applications in a transparent container providing a visual estimate of remaining solution. GnomePen™ contains a unique formulation that is water repellent, insoluble in alcohol and acetone, soluble in xylene and insensitive to detergents (Triton X-100, Tween 20), varying pH and temperature.
Part 6507: GnomePen™ Classic Liquid Blocker: is one of the thinnest PAP pen tips available, allowing for adjacent circles, lines or a drawn pattern to be applied on a single slide. 350 applications.
Part 6508: GnomePen™ Flat Liquid Blocker: produces a wider, flatter line and is optimized for Z-stack confocal microscopy. 250 applications.
METHOD:
Technique: Paraffin, frozen sections and tissue culture cells
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- Manual staining procedures
- Manual immunohistochemistry (IHC) procedures
- Manual immunofluorescence Assay (IFA) procedures
- Confocal microscopy
PROCEDURE:
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- Shake pen thoroughly before use.
- Practice applying GnomePen™ liquid barrier on a test slide; push pen tip against the glass and apply a thin liquid barrier that dries to a film. If flow does not instantly begin, gently squeeze the pen body.
- Store GnomePen™ tightly capped; vertically with cap end up.
- Paraffin Section Method:
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- Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each. Wash well with distilled water.
- Remove slide from water, blot excess solution from slide and tissue section. Or place long edge of slide on absorbent material to remove excess moisture.
- Encircle tissue section(s) on slide surface with GnomePen™ as illustrated. Do not touch the pen to any edges of the tissue.
- The liquid barrier can be drawn on a slightly damp slide.
- See Procedure Notes #1 and #2.
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- Frozen Section and Tissue Culture Cells:
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- Encircle frozen tissue section or tissue culture cells on slide surface at room temperature with the GnomePen™ as illustrated. Do not touch the pen to any edges of the tissue or culture cells.
- See Procedure Notes #1 and #2.
- The liquid barrier should be applied before fixation or prior to the immersion of slide into water or buffer.
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- After liquid barrier application, allow slide to dry in a flat position for 30-60 seconds at room temperature. Proceed with procedure when the drawn barrier has completely dried.
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- See Procedure Note #3.
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- Drain/rinse reagents off between staining steps; blotting slide and tissue as needed to remove excess solution.
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- See Procedure Note #4.
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- Complete staining; coverslip with compatible mounting medium.
- After liquid barrier application, allow slide to dry in a flat position for 30-60 seconds at room temperature. Proceed with procedure when the drawn barrier has completely dried.
PROCEDURE NOTES:
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- Once a tissue section is touched with GnomePen™ liquid barrier it cannot be removed. The section remains useable but colorization will result on the touched tissue.
- If the tissue section is not completely encircled by the GnomePen™ barrier film, reagents will not be fully retained on the section and flood out onto the slide. This may compromise complete and adequate tissue coverage by reagents.
- If GnomePen™ barrier lines are not completely dry prior to staining, a precipitate from reaction with reagents may occur.
- The use of Slide Moisture Chamber (68431, 68432) or StainTray™ (6847, 6848) is recommended for manual staining to maintain slide organization and a moist environment during the procedure.
REFERENCES:
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- Grizzle, William, Cecil Stockard and Paul Billings. “The Effects of Tissue Processing Variables Other Than Fixation on Histochemical Staining and Immunohistochemical Detection of Antigens.” The Journal of Histotechnology 24.3 (2001): 213-219.
- Modifications developed by Newcomer Supply Laboratory.
If you are outside of North America, please visit the Invignome website for a distributor near you.