Pigment, Substance & Mineral Stains
- Shelf Life for most Stain Kits is one year from date manufactured.
- Store Stain Kits at 15-30°C unless otherwise stated.
- Most Stain Kits include 2 Complimentary Positive Control Slides to be used for the initial verification of staining techniques and reagents.
- Stain solution groups are sold as individual products under separate part numbers with storage requirements and expiration date designated per bottle.
- H = Hazardous
For a more detailed chart regarding Pigments & Artifacts, click here.
Name | Comments | Products | Part # |
Copper | Some methods demonstrate copper associated protein rather than copper itself | Copper Stain Kit
Control Slides |
9113 |
Hemosiderin | Prussian Blue reaction stains ferric (+3) ions | Iron Stain Kit
Control Slides |
9136 |
Hematoidin | Similar to bilirubin often formed as a result of hemorrhage, stains with bile methods but not with iron methods | ||
Bile | Demonstrated when bilirubin is oxidized to biliverdin in an acid staining environment | Bilirubin Stain
Control Slides |
Procedure |
Calcium | Black deposits formed when using silver reactions are due to reduction of silver by organic material followed by exposure to strong light | Calcium Stain
Control Slides |
Procedure |
Urates |
Both forms are water soluble, aqueous fixatives should be avoided
Absolute ethanol is the fixative of choice
Gout – monosodium urate crystals appear yellow when their long axes are aligned parallel to a red compensator filter
Pseudogout -calcium pyrophosphate crystals, appear blue when their long axes are aligned parallel to a red compensator filter
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Urates Stain
Control Slides |
Procedure |
Melanin | Identified by a number of methods can interfere with pathology interpretation if found in large amounts | Melanin Stain
Control Slides |
Procedure |
Lipofusin | Stains variably but usually PAS positive | PAS Stain Kit | 9162 |
Artifact:
Carbon | Inert and unreactive, resists removal procedures, commonly found in lung and mediastinal lymph nodes. | |
Melanin | Can become problematic in large amounts and be removed by a variety of methods | Tech Memo |
Formalin | Formed in unbuffered formalin when pH shifts to acidic, not reactive with iron staining methods | Tech Memo |
Mercury | Deposited as a result of using mercury based fixatives | Tech Memo |
Amyloid, Bennhold Congo Red Stain Kit
Identifies the extraneous protein deposits in amyloidosis. The use of polarizing lenses is the essential technique for visualizing amyloid positive areas and/or to confirm negativity.
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- Microwave Modification Included
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Amyloid, Puchtler Congo Red Stain Kit
Identifies the extraneous protein deposits in amyloidosis. The use of polarizing lenses is the essential technique for visualizing amyloid positive areas and/or to confirm negativity.