Hematoxylin & Eosin Staining

 

The routine hematoxylin and eosin (H&E) stain is used for screening specimens in anatomic pathology, as well as for research, smears, touch preps and other applications.  Its two primary coloring agents stain all cellular material including nuclei (blue), and cytoplasmic elements (pink-red).  Popularity of this stain is due, in large measure, to its simplicity, ability to clearly demonstrate a wide variety of different tissue components, dependability, repeatability, and speed of use.

  • H = Hazardous

Bluing Agents

   
 

Scott Tap Water Substitute

Part # 1380A 500 ml $16.00  
Part # 1380B 1 L $25.00  
Part # 1380C 1 gal $36.20  
   
 

Ammonium Hydroxide 28 - 30%, ACS

This product is located in the Chemicals & Reagents Section.

Click Here for part number and pricing.

Reagent should be used per lab protocol as a bluing agent.

   
 

Lithium Carbonate Saturated Aqueous Solution

This product is located in the Chemicals & Reagents Section.

Click Here for part number and pricing.

Reagent should be used per lab protocol as a bluing agent.

Eosins

   
 

Eosin-Phloxine Stain Set

 

Solution A: Eosin Y Stock Stain 1%, Aqueous                1000 ml
Solution B: Phloxine B Stock Stain 1%, Aqueous         100 ml

Part # 1082A set $55.60  
   
 

Eosin Y Stock Stain 1%, Alcoholic

(use: Counterstain for H&E Stain.)

      • Shelf Life is 2 years from date of manufacture.
Part # 1070B 500 ml $22.40 H
Part # 1070C 1 L $29.80 H
Part # 1070D 1 gal $71.80 H
   
 

Eosin Y Stock Stain 1%, Aqueous

(use: Counterstain for H&E Stain.)

Part # 1080B 500 ml $14.40  
Part # 1080C 1 L $19.80  
Part # 1080D 1 gal $59.20  
   
 

Eosin Y Working Solution

(use: Counterstain for H&E Stain.)

Part # 1072A 500 ml $17.10 H
Part # 1072B 1 L $28.40 H
Part # 1072C 1 gal $54.00 H
   
 

Eosin Y, Powder

CI 45380

      • Shelf Life is 4 years from date of manufacture.
Part # 1060A 25 g $24.20  
Part # 1060B 100 g $78.50  

Hematoxylins

   
 

Hematoxylin Stain, Harris Modified

Ready to use, doesn’t require filtering, is enhanced with glacial acetic acid along with ethylene glycol and is mercury free.

      • Shelf Life is 2 years from date of manufacture.
Part # 1201A 500 ml $36.30  
Part # 1201B 1 L $58.60  
Part # 1201C 1 gal $133.30  
   
 

Hematoxylin Stain, Harris

Ready to use, doesn’t require filtering and is mercury free.

      • Shelf Life is 2 years from date of manufacture.
Part # 12013A 500 ml $38.70  
Part # 12013B 1 L $61.00  
Part # 12013C 1 gal $138.70  
   
Newly Blue Stain Set - Hematoxylin Alternative

Newly Blue Stain Set

(use: Hematoxylin replacement stain.)

Solution A: Celestine Blue Stain 1%, Aqueous            250ml
Solution B: Ferric Ammonium Sulfate 4%, Aqueous    250ml

Part # 1258A 500 ml set $30.00  
   
 

Hematoxylin Stain, Gill

Uses:

      • Gill I – Cytology
      • Gill II – Frozen & paraffin sections & IHC
      • Gill III – Frozen sections & IHC darker staining & high volume.
Gill I (Cytology):
Part # 1180A 500 ml $27.20  
Part # 1180C 1 gal $109.10  
Gill II (Cytology & Histology):
Part # 1180D 500 ml $30.00  
Part # 1180F 1 gal $128.90  
Gill III (Histology):
Part # 1180G 500 ml $33.50  
Part # 1180I 1 gal $144.00  
   
 

Hematoxylin Stain, Mayer Modified

No Chloral Hydrate. Excellent for IHC’s.  Nuclear staining for non acidic special stains.

      • Shelf Life is 2 years from date of manufacture.
Part # 1202A 500 ml $36.70  
Part # 1202B 1 L $57.90  
Part # 1202C 4 L $161.30  
   
 

Hematoxylin, Powder

CI 75290

      • Shelf Life is 4 years from date of manufacture.
Part # 1160A 10 g $67.10  
Part # 1160B 25 g $154.30  
Part # 1160C 100 g $226.30  
   
Hematoxylin Stain Set, Weigert Iron

Hematoxylin Stain Set, Weigert Iron

(use: Mucin & Trichrome.)

        • Shelf Life is 2 years from date of manufacture.

Solution A: Ferric Chloride, Acidified                             250 ml or 500 ml
Solution B: Hematoxylin 1%, Alcoholic                          250 ml or 500 ml

(Nuclear counterstain for acidic special stains)

each bottle 250 ml:
Part # 1409B 500 ml set $88.50 H
each bottle 500 ml:
Part # 1409A 1 L set $118.50 H