Hematoxylin Stain, Harris Modified

Ready to use, doesn’t require filtering, is enhanced with glacial acetic acid along with ethylene glycol and is mercury free.

  • Shelf Life is 2 years from date of manufacture.

 

Product Options:

Part # 1201A 500 ml $35.10
Part # 1201B 1 L $55.30
Part # 1201C 1 gal $125.70

STAIN SOLUTION:                                                                                      

Part 1201A Part 1201B Part 1201C
Hematoxylin Stain, Harris Modified 500ml 1 Liter 1 Gallon

Additionally Needed For H&E Staining:

Hematoxylin and Eosin (H&E) Control Slides Part 4278
Xylene, ACS Part 1445
Alcohol, Ethyl Denatured, 100% Part 10841
Alcohol, Ethyl Denatured, 95% Part 10842
Acid Alcohol 1% Part 10011
Lithium Carbonate, Saturated Aqueous

                   or

Scott Tap Water Substitute

Part 12215

      or

Part 1380

Alcohol, Ethyl Denatured, 70% Part 10844
Eosin Y Working Solution

               or

Eosin-Phloxine Stain Set

Part 1072

     or

Part 1082

For storage requirements and expiration date refer to individual product labels.

 

APPLICATION:

Newcomer Supply Hematoxylin Stain, Harris Modified is a ready to use regressive hematoxylin that does not require filtering, is mercury-free and can be used in either manual or automated staining platforms. This modified Harris formulation contains glacial acetic acid for more precise nuclear staining and ethylene glycol to increase solution stability and reduce surface precipitate.

Hematoxylin and eosin (H&E) staining is used for screening specimens in anatomic pathology, for research, smears, touch preps and other applications.  Its two primary coloring agents stain all cellular material; nuclei (blue), and cytoplasmic elements (pink-red).  Popularity of this stain is due to its simplicity, ability to clearly demonstrate a variety of tissue components, dependability, repeatability, and speed of use.

Quality Control: Since hematoxylin and eosin staining is the foundation of the diagnostic process, maintaining quality is of critical importance. Procedures will vary between laboratories depending upon volume of slides, automation vs manual staining, chemical hygiene and solution integrity.  Longevity of hematoxylin depends upon these factors and stain quality should be regularly screened with an H&E control slide.

 

METHOD:

Fixation:  Formalin 10%, Phosphate Buffered (Part 1090)

Technique:  Paraffin sections cut at 4 microns

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

H&E STAINING PROCEDURE WITH HARRIS MODIFIED:

    1. If necessary, heat dry tissue sections/slides in oven.
    2. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
        1. See Procedure Notes #1 and #2.
    3. Stain with Hematoxylin Stain, Harris Modified, 1 to 5 minutes, depending on preference of nuclear stain intensity.
    4. Wash well in three changes of tap water.
    5. Differentiate quickly in Acid Alcohol 1% (Part 10011).
        1. Nuclei should be distinct and background very light to colorless.
    6. Rinse well in three changes of tap water.
    7. Blue in Lithium Carbonate, Saturated Aqueous (Part 12215) or Scott Tap Water Substitute (Part 1380) for 10 dips.
    8. Wash in three changes of tap water; rinse in distilled water.
    9. Drain excess water; proceed to 70% alcohol for 10 dips.
    10. Counterstain in Eosin Y Working Solution (Part 1072) or prepared Eosin-Phloxine Working Solution (Part 1082) for 30 seconds to 3 minutes, depending on preference of intensity.
    11. Dehydrate in two changes of 95% ethyl alcohol for 1 minute each and two changes of 100% ethyl alcohol, 10 dips each.   Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.

 

RESULTS:

Nuclei Blue
Erythrocytes and eosinophilic granules Bright pink to red
Cytoplasm and other tissue elements Various shades of pink

 

PROCEDURE NOTES:

    1. Drain slides after each step to prevent solution carry over.
    2. Do not allow sections to dry out at any point during procedure.
    3. Store hematoxylin at room temperature in tightly capped container away from direct light to minimize oxidation and extend shelf life.
    4. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.

 

REFERENCES:

    1. Bancroft, John D., and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 123-125.
    2. Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 113-114, 118-120.
    3. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 142-144, 153-154.
    4. Modifications developed by Newcomer Supply Laboratory.