Products

Mucin Mucicarmine

Mucin Mucicarmine Stained Histology Slide

Validation Stain: Mucin Mucicarmine

 

Tissue: 
(+) Colon
Adhesive or Charged Slides: 
Superfrost +

Product Options:

Part # 4455A 10/set $60.80
Part # 4455B 98/set $452.40

PRODUCT SPECIFICATIONS:

Tissue:  Positive staining colon.
Fixation: Formalin 10%, Phosphate Buffered (Part 1090).
Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.
Quality Control Stain:  Mayer Mucicarmine quality control stained slide(s) included.
Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity. 
Storage: 15-30°C in a light deprived and humidity controlled environment.
Intended Use: To verify histological techniques and reagent reactivity.

Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.

 

CONTROL SLIDE VALIDATION:

With Mucin, Mayer Mucicarmine Stain Kit: Part 9151A/B Individual Stain Solution
Solution A: Ferric Chloride, Aqueous 125/250 ml Part 1409
Solution B: Hematoxylin 1%, Alcoholic 125/250 ml Part 1409
Solution C: Mucicarmine Stock Stain, Mayer 125/125 ml Part 1250
Solution D: Metanil Yellow Stain, Aqueous 250/500 ml Part 12235

 

APPLICATION:

Newcomer Supply Mucin Mucicarmine Control Slides are for the positive histochemical staining of acid epithelial mucins (sialomucin, sulfomucin).

 

NEWCOMER SUPPLY VALIDATION PROCEDURE:

    1. Heat dry sections in oven according to your laboratory protocol.
    2. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
        1. See Procedure Notes #1 and #2.
    3. Prepare fresh Weigert Iron Hematoxylin Working Solution directly before use; combine and mix well:
        1. Solution A: Ferric Chloride, Aqueous   20 ml
        2. Solution B: Hematoxylin 1%, Alcoholic             20 ml
    4. Stain in fresh Weigert Iron Hematoxylin Working Solution for 7 minutes.
    5. Rinse in running tap water for 10 minutes.
    6. Prepare fresh Mayer Mucicarmine Working Solution; combine and mix well:
        1. Solution C: Mucicarmine Stock Stain, Mayer   10 ml  
        2. Tap Water (do not use distilled water)   30 ml    
    7. Stain in fresh Mayer Mucicarmine Working Solution for 60 minutes or longer if a more intense stain is desired.

Microwave Modification:  See Procedure Note #3.

        1. Place slides in a plastic Coplin jar containing fresh Mayer Mucicarmine Working Solution. Microwave for 10 minutes at 70°C.
    1. Rinse in several changes of tap water.
    2. Counterstain in Solution D: Metanil Yellow Stain, Aqueous for 30 to 60 seconds.
    3. Dehydrate quickly through 95% and 100% ethyl alcohols. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.

 

RESULTS:

Acid epithelial mucins Deep rose to red
Nuclei Black
Other tissue elements Yellow

 

PROCEDURE NOTES:

    1. Drain slides after each step to prevent solution carry over.
    2. Do not allow sections to dry out at any point during procedure.
    3. The microwave procedure was tested using a laboratory-grade microwave oven. This procedure is a guideline and techniques should be developed for use in your laboratory.
    4. If using a xylene substitute, follow manufacturer’s recommendation for deparaffinization and clearing steps.

 

REFERENCES:

    1. Bancroft, John D. and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 174-175.
    2. Carson, Freida L. and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 142-144.
    3. Sheehan, Dezna C. and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 168-169.
    4. Modifications developed by Newcomer Supply Laboratory.