Mucin, Mayer Mucicarmine Stain Kit

Stains acid epithelial mucin (sialomucin, sulfomucin) and is also useful for the demonstration of the encapsulated yeast Cryptococcus neoformans.

Product Options:

Part # 9151A 250ml kit $153.50 H
Part # 9151B 500ml kit $231.30 H

 

  

MUCIN, MAYER MUCICARMINE STAIN KIT INCLUDES:

Part 9151A Part 9151B
Solution A: Ferric Chloride, Acidified 125 ml 250 ml
Solution B: Hematoxylin 1%, Alcoholic 125 ml 250 ml
Solution C: Mucicarmine Stock Stain, Mayer 125 ml 125 ml
Solution D: Metanil Yellow Stain, Aqueous 250 ml 500 ml

 

COMPLIMENTARY POSITIVE CONTROL SLIDES: Enclosed are two complimentary unstained positive control slides for the initial verification of staining techniques and reagents.  Verification must be documented by running one Newcomer Supply complimentary positive control slide along with your current positive control slide for the first run. Retain the second complimentary control slide for further troubleshooting, if needed.

Individual stain solutions and additional control slides may be available for purchase under separate part numbers.

 

Additionally Needed:

Xylene, ACS Part 1445
Alcohol, Ethyl Denatured, 100% Part 10841
Alcohol, Ethyl Denatured, 95% Part 10842
Coplin Jar, Plastic Part 5184 (for microwave modification)

For storage requirements and expiration date refer to individual bottle labels. 

 

APPLICATION:

Newcomer Supply Mucin, Mayer Mucicarmine Stain Kit procedure, with included microwave modification, is used to stain acid epithelial mucin (sialomucin, sulfomucin) and is also useful for the demonstration of the encapsulated yeast Cryptococcus neoformans.

 

METHOD:

Fixation: Formalin 10%, Phosphate Buffered (Part 1090)

Technique:  Paraffin sections cut at 4 microns

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

All Newcomer Supply Stain Kits are designed to be used with Coplin jars filled to 40 ml following the staining procedure provided below.  Some solutions in the kit may contain extra volumes.

 

STAINING PROCEDURE:

    1. If necessary, heat dry tissue sections/slides in oven.
    2. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
        1. See Procedure Notes #1 and #2.
    3. Prepare fresh Weigert Iron Hematoxylin Working Solution directly before use; combine and mix well.
        1. Solution A: Ferric Chloride, Acidified   20 ml
        2. Solution B: Hematoxylin 1%, Alcoholic             20 ml
    4. Stain in fresh Weigert Iron Hematoxylin Working Solution for 7 minutes.
    5. Rinse in running tap water for 10 minutes.
    6. Prepare fresh Mayer Mucicarmine Working Solution; combine and mix well.
        1. Solution C: Mucicarmine Stock Stain, Mayer   10 ml   
        2. Tap Water (do not use distilled water)   30 ml    
    7. Stain slides in fresh Mayer Mucicarmine Working Solution for 60 minutes or longer if a more intense stain is desired.

Microwave Modification:  See Procedure Note #3.

        1. Place slides in a plastic Coplin jar containing fresh Mayer Mucicarmine Working Solution and microwave at 70°C for 10 minutes.
    1. Rinse in several changes of tap water.
    2. Counterstain in Solution D: Metanil Yellow Stain, Aqueous for 30 seconds to 1 minute.
    3. Dehydrate quickly through 95% and 100% ethyl alcohols. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.

                                                                                                                                                                              

RESULTS:

Acid epithelial mucin Deep rose to red
Capsule of Cryptococcus neoformans Deep rose to red
Nuclei Black
Other tissue elements Yellow

 

PROCEDURE NOTES:

    1. Drain slides after each step to prevent solution carry over.
    2. Do not allow sections to dry out at any point during procedure.
    3. The suggested microwave procedure has been tested at Newcomer Supply. This procedure is a guideline and techniques should be developed for use in your laboratory.
    4. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.

 

REFERENCES:

    1. Bancroft, John D., and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 174-175.
    2. Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 142-144.
    3. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 168-169.
    4. Modifications developed by Newcomer Supply Laboratory.