Helicobacter sp., Artificial
Validation Stain: Differential
Other Applicable Stains: Warthin Starry, Steiner-Steiner Modified Silver, Steiner-Chapman Modified Silver and Modified Giemsa
Recommended for use with Differential Stain.
(Not in human tissue.) Organisms artificially introduced into a rat organ (e.g., lung) through a non-infective process.
Tissue: Positive staining rat lung.
Fixation: Formalin 10%, Phosphate Buffered (Part 1090).
Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.
Quality Control Stain: Thiazine quality control stained slide(s) included.
Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity.
Storage: 15-30°C in a light deprived and humidity controlled environment.
Intended Use: To verify histological techniques and reagent reactivity.
Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.
CONTROL SLIDE VALIDATION:
|With Differential Stain, Monochromatic Method:||Individual Stain Solution|
|Thiazine Stain||Part 10522|
Newcomer Supply Helicobacter sp., Artificial Control Slides are for the positive histochemical staining of Helicobacter sp., a spiral shaped bacterium associated with stomach inflammation and implicated in the development of gastric malignancy, peptic ulcers, and chronic gastritis.
Helicobacter pylori purchased from American Type Culture Collection is used to produce the positive control tissue.
NEWCOMER SUPPLY VALIDATION PROCEDURE:
- Heat dry sections in oven according to your laboratory protocol.
- Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each. Wash well with distilled water.
- Place slides in Thiazine Stain for 1-4 minutes depending upon staining intensity preference.
- See Procedure Note #1.
- Rinse slides quickly in distilled water; long enough to remove excess stain.
- Allow slides to air-dry in a vertical position.
- Dip dried slides in xylene and coverslip with compatible mounting medium.
- See Procedure Note #2.
|Helicobacter sp.||Dark blue|
|Collagen and muscle||Blue|
|Cytoplasm||Varying shades of light blue|
- The timings provided are suggested ranges. Optimal staining times will depend upon staining intensity preference.
- The elimination of dehydration steps is necessary to retain the dark stain of the organism.
- If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and coverslipping steps.
- Potvin, Carol. “A Modified Diff-Quik Stain for Helicobacter pylori in Gastrointestinal Biopsies.” Laboratory Medicine 25.6 (1994): 389-391.
- Skipper, Ray, and Don DeStephano. “A Rapid Stain for Campylobacter pylori in Gastrointestinal Tissue Sections Using Diff-Quik.” The Journal of Histotechnology 12.4 (1989): 303-304.
- Modifications developed by Newcomer Supply Laboratory.