Products

Decolorizer, Alcoholic

Newcomer Supply Beaker

Product Options:

Part # 1046A 500 ml $62.70 H
Part # 1046B 1 L $78.40 H
Part # 1046C 1 gal $103.50 H

SOLUTION:

Part 1046A Part 1046B Part 1046C
Decolorizer, Alcoholic 500 ml 1 Liter 1 Gallon

 

Additionally Needed For H&E Staining:

Hematoxylin and Eosin (H&E) Control Slides Part 4278
Xylene, ACS Part 1445
Alcohol, Ethyl Denatured, 100% Part 10841
Alcohol, Ethyl Denatured, 95% Part 10842
Hematoxylin Stain, Harris Modified Part 1201
Lithium Carbonate, Saturated Aqueous
                 OR
Scott Tap Water Substitute		 Part 12215
    OR
Part 1380
Alcohol, Ethyl Denatured, 70% Part 10844
Eosin Y Working Solution
                OR
Eosin Y-Phloxine B Working Solution		 Part 1072
    OR
Part 1083

 

For storage requirements and expiration date refer to individual product labels.

 

APPLICATION:

Newcomer Supply Decolorizer, Alcoholic is a ready to use solution to improve staining contrast between nuclei and cytoplasm in automated regressive hematoxylin and eosin (H&E) stain protocols.  Decolorizer, Alcoholic works to slowly remove the correct amount of hematoxylin without concerns for over differentiation in automated H&E staining systems.  For manual H&E staining, Decolorizer, Alcoholic can be implemented for a more controlled differentiation step.

 

METHOD:

Fixation:  Formalin 10%, Phosphate Buffered (Part 1090)
Technique:  Paraffin sections cut at 4 microns
Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

H&E AUTOMATED STAINING WITH DECOLORIZER, ALCOHOLIC:

    1. If necessary, heat dry tissue sections/slides in oven or heat station.
    2. Deparaffinize and hydrate sections in the suggested number of stations and corresponding timings for xylenes and alcohols as specified per the automated stainer protocol.
    3. Wash well in distilled water.
    4. Stain with Hematoxylin Stain, Harris Modified (Part 1201), 2 to 5 minutes, depending on preference of nuclear stain intensity.
    5. Wash well in tap water.
    6. Differentiate in Decolorizer, Alcoholic for 15 to 45 seconds.
        1. Timing depends on length of hematoxylin stain in Step #4.
        2. See procedure note #1.
    7. Rinse well in tap water.
    8. Blue in Lithium Carbonate, Saturated Aqueous (Part 12215) or Scott Tap Water Substitute (Part 1380) for 60 to 90 seconds.
    9. Wash well in tap water.
    10. Proceed to alcohol rinse for 1 minute.
        1. Use suggested percentage of alcohol per stainer protocol.
    11. Counterstain in Eosin Y Working Solution (Part 1072) or Eosin Y-Phloxine B Working Solution (Part 1083) for 30 seconds to 3 minutes, depending on preference of cytoplasmic stain intensity.
    12. Dehydrate and clear in the suggested number of stations and corresponding timings for alcohols and xylenes as specified per automated stainer protocol.
    13. Coverslip with compatible mounting medium.

 

RESULTS:

Nuclei Blue
Erythrocytes and eosinophilic granules Bright pink to red
Cytoplasm and other tissue elements Various shades of pink

 

PROCEDURE NOTES:

    1. For manual H&E staining, follow regressive H&E stain protocol;
        1. Use Decolorizer, Alcoholic in the hematoxylin differentiation step for 15 to 45 seconds.
        2. Timing will depend on length of hematoxylin stain exposure.
        3. Proceed through completion of the stain.
    2. If using a xylene substitute, follow manufacturer’s recommendation for deparaffinization and clearing steps.

 

REFERENCES:

    1. Carson, Freida L. and Christa Cappellano. Histotechnology: A Self-Instructional Text. 5th ed. Chicago: ASCP Press, 2020. 122-125.
    2. Suvarna, S. Kim., Christopher Layton, and John D. Bancroft. Bancroft’s Theory and Practice of Histological Techniques. 8th ed. Oxford: Elsevier, 2019. 145-147.
    3. Modifications developed by Newcomer Supply Laboratory.