Fite, Leprosy, Animal

Tissue: 
(+) Animal Spleen (-) Lung
Adhesive or Charged Slides: 
Superfrost +

Product Options:

Part # 4212A 10/set $90.00
Part # 4212B 98/set $720.00

 

PRODUCT SPECIFICATIONS:

Tissue:  Positive staining animal spleen and negative staining human lung.

Fixation: Formalin 10%, Phosphate Buffered (Part 1090).

Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.

Quality Control Stain:  AFB, Fite quality control stained slide(s) included.

Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity. 

Storage: 15-30°C in a light deprived and humidity controlled environment.

Intended Use: To verify histological techniques and reagent reactivity.

Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.

CONTROL SLIDE VALIDATION:

With AFB, Fite Stain Kit: Part 91013A Individual Stain Solution
Solution A:  Xylene/Peanut Oil, 2:1 500ml Part 1449
Solution B:  Carbol Fuchsin Stain, Ziehl-Neelsen 250ml Part 1030
Solution C:  Acid Alcohol 1% 250ml Part 10011
Solution D:  Light Green SF Yellowish 0.1%, Aqueous 250ml Part 12203

 

APPLICATION:

Newcomer Supply Fite, Leprosy, Animal Control Slides are for the positive histochemical staining of Mycobacterium leprae sp., the causative agent of leprosy.

PRESTAINING PREPARATION:

    1. Heat dry sections in oven according to your laboratory protocol.
    2. Filter Solution B: Carbol Fuchsin Stain, Ziehl-Neelsen with high quality filter paper.

 

NEWCOMER SUPPLY VALIDATION PROCEDURE:

    1. Deparaffinize slides in Solution A: Xylene/Peanut Oil, 2:1, two changes for 10 minutes each.
        1. See Procedure Note #1.
    2. Drain slides, wipe off excess oil, and blot to opacity taking care to remove residual oil.
        1. See Procedure Note #2.
    3. Stain in freshly filtered Solution B: Carbol Fuchsin Stain, Ziehl-Neelsen for 15 minutes at room temperature.
    4. Rinse well in distilled water.
    5. Differentiate slides individually in Solution C: Acid Alcohol 1% until sections are light pink; 5-10 dips.
    6. Rinse well in distilled water.
    7. Counterstain in Solution D: Light Green SF Yellowish 0.1%, Aqueous; 5-10 dips.
    8. Rinse in distilled water.
    9. Blot excess water from slide and air-dry or oven-dry completely.
    10. Dip dried slides in xylene and coverslip with a compatible mounting medium.

 

RESULTS:

leprae Red
Other tissue elements Green
Negative lung Negative for Mycobacterium leprae

 

PROCEDURE NOTES:

    1. Acid-fastness of leprosy organisms is enhanced when the waxy capsule is protected by the mixture of xylene/peanut oil and avoidance of dehydrating solutions.
    2. It is important to blot well, residual oil may produce staining artifact.
    3. If using a xylene substitute, closely follow the manufacturer’s recommendations for coverslipping step.

 

REFERENCES:

    1. Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 220-221.
    2. Fite, George, P.J. Cambre and M.H. Turner. “Procedure for Demonstrating Lepra Bacilli in Paraffin Sections”. Archives of Pathology 43 (1947). 624-625.
    3. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 237.
    4. Modifications developed by Newcomer Supply Laboratory.