Light Green SF Yellowish Stain 0.1%, Aqueous
(use: Counterstain for fungus stain PAS Light Green)
|250 ml||500 ml|
|Light Green SF Yellowish Stain 0.1%, Aqueous||Part 12203A||Part 12203B|
|Fungus, PAS, Aspergillus sp., Artificial Control Slides
Fungus, PAS, Candida sp., Artificial Control Slides
|Periodic Acid 0.5%, Aqueous||Part 13308|
|Schiff Reagent, McManus||Part 1371|
|Xylene, ACS||Part 1445|
|Alcohol, Ethyl Denatured, 100%||Part 10841|
|Alcohol, Ethyl Denatured, 95%||Part 10842|
For storage requirements and expiration date refer to individual product labels.
Newcomer Supply Light Green SF Yellowish 0.1%, Aqueous provides a complimentary counterstain for the Fungus/PAS Stain.
Fixation: Formalin 10%, Phosphate Buffered (Part 1090)
Technique: Paraffin section cut at 4 microns
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
All Newcomer Supply stain procedures are designed to be used with Coplin jars filled to 40 ml following the provided staining procedure.
- If necessary, heat dry tissue sections/slides in oven.
- Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each. Wash well with distilled water.
- See Procedure Notes #1 and #2.
- Place in Periodic Acid 0.5%, Aqueous (Part 13308) for 5 minutes.
- Wash in three changes of tap water; rinse in distilled water.
- Drain slides of excess water and stain in Schiff Reagent, McManus (Part 1371) for 20 minutes.
- Wash gently in lukewarm tap water for 10 minutes to allow pink color to develop.
- Counterstain in Light Green SF Yellowish Stain 0.1%, Aqueous for 5 seconds.
- See Procedure Note #3.
- Dehydrate in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.
|Fungal cell walls and glycogen||Red to magenta|
- Drain slides after each step to prevent solution carry over.
- Do not allow sections to dry out at any point during procedure.
- Increase or decrease staining time in Light Green SF Yellowish Stain 0.1%, Aqueous for preference of counterstain intensity.
- If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.
- Bancroft, John D., and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 321-323.
- Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 245.
- Modifications developed by Newcomer Supply Laboratory.