Fast Green Stain 2.5%, Aqueous

Newcomer Supply Beaker

Product Options:

Part # 10852A 250 ml $53.80
Part # 10852B 500 ml $82.10


250 ml 500 ml
Fast Green Stain 2.5%, Aqueous Part 10852A Part 10852B


Additionally Needed:

Trichrome, Liver Control Slides
Trichrome, Multi-Tissue Control Slides
Part 4690
Part 4693
Xylene, ACS Part 1445
Alcohol, Ethyl Denatured, 100% Part 10841
Alcohol, Ethyl Denatured, 95% Part 10842
Bouin Fluid Part 1020
Hematoxylin Stain Set, Weigert Iron Part 1409
Biebrich Scarlet-Acid Fuchsin Stain, Aqueous Part 10161
Phosphotungstic Acid 5%, Aqueous Part 13345
Acetic Acid 0.5%, Aqueous Part 100121
Coplin Jar, Plastic Part 5184 (for microwave modification)

For storage requirements and expiration date refer to individual product labels.



Newcomer Supply Trichrome Stain, Masson, Fast Green procedure, with included microwave modification, is used to differentially demonstrate connective tissue elements, collagen and muscle fibers.



Fixation: Formalin 10%, Phosphate Buffered (Part 1090)
Technique:  Paraffin sections cut at 4 microns

    1. See Procedure Note #1.

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

All Newcomer Supply stain procedures are designed to be used with Coplin jars filled to 40 ml following the staining procedure.



    1. If necessary, heat dry tissue sections/slides in oven.
    2. Preheat Bouin Fluid (Part 1020) to 56-60°C in oven or water bath. (Skip if using overnight method or microwave procedure.)



    1. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
        1. See Procedure Notes #2 and #3.
    2. Mordant in preheated Bouin Fluid (Step #2) for one hour at 56-60°C or overnight at room temperature. Cool at room temperature for 5-10 minutes.
        1. Skip Step #4 if tissue was originally Bouin fixed.

       Microwave Modification:  See Procedure Note #4.

        1. Place slides in a plastic Coplin jar containing Bouin Fluid and microwave for 5 minutes at 60° Allow slides to sit an additional 10 minutes in solution.
    1. Wash well in running tap water; rinse in distilled water.
    2. Prepare fresh Weigert Iron Hematoxylin (Part 1409); combine and mix well.
        1. Solution A: Ferric Chloride, Acidified 20 ml
        2. Solution B: Hematoxylin 1%, Alcoholic 20 ml
    3. Stain slides in fresh Weigert Iron Hematoxylin for 10 minutes.
    4. Wash in running tap water for 10 minutes; rinse in distilled water.
        1. See Procedure Note #5.
    5. Place slides in Biebrich Scarlet-Acid Fuchsin Stain, Aqueous (Part 10161) for 2 minutes.
    6. Rinse in distilled water.
    7. Place slides in Phosphotungstic Acid 5%, Aqueous (Part 13345) for 5 minutes.
    8. Transfer slides directly into Fast Green Stain 2.5%, Aqueous for 5-6 minutes, depending on stain intensity preference.
    9. Rinse in distilled water.
    10. Place slides in Acetic Acid 0.5%, Aqueous (Part 100121) for 2 quick dips.
    11. Dehydrate in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.



Collagen and mucin Green
Muscle fibers, cytoplasm and keratin Red
Nuclei Blue/black



    1. Using ammonium hydroxide to soak/face tissue blocks will alter the pH of tissue sections and diminish trichrome staining.
    2. Drain slides after each step to prevent solution carry over.
    3. Do not allow sections to dry out at any point during procedure.
    4. The suggested microwave procedure has been tested at Newcomer Supply. This procedure is a guideline and techniques should be developed for your laboratory.
    5. If Weigert Iron Hematoxylin is not completely washed from tissue sections, nuclear and cytoplasmic staining will be compromised.
    6. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.



    1. Brown, Richard. Histologic Preparations: Common Problems and Their Solutions. Northfield, Ill.: College of American Pathologists, 2009. 95-101.
    2. Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 162-165.
    3. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 191-192.
    4. Vacca, Linda L. Laboratory Manual of Histochemistry. New York: Raven Press, 1985. 308-310.
    5. Modifications developed by Newcomer Supply Laboratory.