Products

Bouin Fluid

Newcomer Supply Beaker

      • Shelf Life is 2 years from date of manufacture.

Product Options:

Part # 1020A 1 L $40.00
Part # 1020B 1 gal $81.00
Part # 1020C 20 L cube $180.00

SOLUTION:

1 Liter 1 Gallon 20 Liter Cube
Bouin Fluid Part 1020A Part 1020B Part 1020C

 

For storage requirements and expiration date refer to individual bottle labels.

 

APPLICATION:

Newcomer Supply Bouin Fluid is a ready-to-use picric acid based tissue fixative combined with acetic acid and formalin.  Bouin Fluid penetrates rapidly, fixes evenly, provides crisp nuclear staining and preserves structures with soft and delicate features.

Bouin Fluid is recommended for a variety of specimens including bone marrow clots and biopsies, gastrointestinal tract biopsies, testicular biopsies and lymph nodes.  It also serves as both fixative and mordant for tissues stained with trichrome procedures.

 

METHOD:

Fixation Recommendations:

    • Bone Marrow Clots/Biopsy: 4 hours to 24 hours.
    • Lymph Nodes: Up to 24 hours.
    • Small nodes (5 mm or less) should be halved.
    • Large nodes dissected with no piece thicker than 2-3 mm.
    • Small Biopsies: 4 hours to 24 hours.
    • Fatty Tissue and Lipomas: 48 to 72 hours.

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

FIXATION PROCEDURE:

    1. Place fresh tissue directly in Bouin Fluid after surgical excision.
        1. See Procedure Notes #1 and #2.
    2. Hold tissue in Bouin Fluid for processing or a maximum of 72 hours.
        1. See Procedure Note #3.
    3. Rinse Bouin fixed tissue thoroughly in running tap water followed by 15 minute minimum wash in 70% ethyl alcohol (Part 10844) prior to processing.
    4. Place on tissue processor in Formalin 10%, Phosphate Buffered (Part 1090) fixation step.
    5. Blocked and sectioned tissues may retain excess picric acid pigment. The yellow picric acid color will normally be removed from tissue sections in the deparaffinization process. Additional methods of removing picric acid are:
        1. Wash deparaffinized tissue sections in running tap water or 70% ethyl alcohol until yellow pigment is removed.
        2. Rinse deparaffinized tissue sections in 70% ethyl alcohol saturated with lithium carbonate until yellow pigment is removed.
    6. Post-fixation applications of Bouin Fluid include use as a mordant to intensify color reactions in trichrome staining procedures.
        1. Refer to trichrome stain protocols for more information.

 

PROCEDURE NOTES:

    1. If received in Formalin 10%, Phosphate Buffered, rinse tissue thoroughly in tap water prior to placing in Bouin Fluid.
    2. Bouin Fluid should not be used for preservation of red blood cells, tissues for electron microscopy or for nuclei acid demonstration.
    3. Extended storage in Bouin Fluid is not recommended
        1. After maximum fixation, rinse well in running tap water and transfer Bouin fixed tissue to 70% ethyl alcohol or Formalin 10%, Phosphate Buffered for long-term storage purposes.
    4. Collect Bouin Fluid waste solutions in an appropriately labeled leak proof container for proper disposal.
        1. Do not use metal containers.
        2. Do not use containers with a metal cap or lid.
        3. Do not dispose Bouin Fluid down the drain.

 

REFERENCES:

    1. Carson, Freida L. and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 19-20.
    2. Dapson, Janet Crookham and Richard Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling, and Disposal. 4th ed. Battle Creek, MI: Anatech, 2005. 150, 265-266.
    3. Sheehan, Dezna C. and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 43, 47, 50.
    4. Modifications developed by Newcomer Supply Laboratory.