Aminoalkylsilane Slide Adhesive
Sections stuck for good!
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- Shelf Life is 6 months from date of manufacture.
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SOLUTION:
250 ml | 500 ml | 1 Liter | |
Aminoalkylsilane Slide Adhesive | Part 1007A | Part 1007B | Part 1007C |
For storage requirements and expiration date refer to individual bottle labels.
APPLICATION:
Newcomer Supply Aminoalkylsilane Slide Adhesive is a working adhesive solution used to treat non-adhesive glass microscope slides and provide exceptionally strong tissue adhesion properties for paraffin and frozen tissue sections, while leaving very minimal or no background staining.
METHOD:
Technique: Paraffin or frozen sections
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
PROCEDURE:
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- Fill slide rack(s) with clean dry slides.
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- If necessary, clean racked slides in 4-5 dips of Acetone (Part 10014) prior to treating.
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- Shake bottle of Aminoalkylsilane Slide Adhesive before use. Pour solution into an appropriate size staining dish.
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- Use sufficient solution to completely cover slides.
- Keep solution covered to avoid evaporation.
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- Soak slides in the Aminoalkylsilane Slide Adhesive for 2 minutes.
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- See Procedure Notes #1 and #2.
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- Rinse slides well in three changes of distilled water; 5 dips each.
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- Thorough rinsing removes excess adhesive and reduces occurrence of background staining.
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- Drain slides. Blot and tap excess water to prevent water spotting.
- Dry slides in a 60°C oven for a minimum of 30 minutes or overnight at room temperature.
- Store dried treated slides in a clean slide box at room temperature.
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- Slides will adhere together if not thoroughly dried before storing.
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- Wash emptied slide racks after use to ensure adhesive is removed.
- Fill slide rack(s) with clean dry slides.
PROCEDURE NOTES:
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- Store used Aminoalkylsilane Slide Adhesive in a separate well sealed container; reuse for up to three weeks.
- 250 ml of Aminoalkylsilane Slide Adhesive will treat 400 slides.
- Aminoalkylsilane Slide Adhesive is initially clear or light amber in color and may become cloudy and turn darker in color as it ages.
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- Adhesive effectiveness will not diminish if these changes occur.
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- Tissue section will strongly adhere to treated slide from first contact, position section carefully.
- To prevent formation of water blisters trapped between paraffin section and glass, drain and dry sectioned slides in a vertical position.
REFERENCES:
- Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 70.
- Henderson, Colin. “Aminoalkylsilane: An Inexpensive Simple Preparation for Slide Adhesion.” The Journal of Histotechnology2 (1989): 123-124.
- Modifications developed by Newcomer Supply Laboratory.