Aminoalkylsilane Slide Adhesive

Sections stuck for good! Shelf Life 6 mos.

Product Options:

Part # 1007A 250 ml $29.40 H
Part # 1007B 500 ml $38.90 H
Part # 1007C 1L $52.30 H

 

 

SOLUTION:

250 ml  500 ml 1 Liter
Aminoalkylsilane Slide Adhesive Part 1007A Part 1007B Part 1007C

 

For storage requirements and expiration date refer to individual bottle labels.

 

APPLICATION:

Newcomer Supply Aminoalkylsilane Slide Adhesive is a working adhesive solution used to treat non-adhesive glass microscope slides and provide exceptionally strong tissue adhesion properties for paraffin and frozen tissue sections, while leaving very minimal or no background staining.

 

METHOD:

Technique:  Paraffin or frozen sections

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

PROCEDURE:

    1. Fill slide rack(s) with clean dry slides.
        1. If necessary, clean racked slides in 4-5 dips of Acetone (Part 10014) prior to treating.
    2. Shake bottle of Aminoalkylsilane Slide Adhesive before use. Pour solution into an appropriate size staining dish.
        1. Use sufficient solution to completely cover slides.
        2. Keep solution covered to avoid evaporation.
    3. Soak slides in the Aminoalkylsilane Slide Adhesive for 2 minutes.
        1. See Procedure Notes #1 and #2.
    4. Rinse slides well in three changes of distilled water; 5 dips each.
        1. Thorough rinsing removes excess adhesive and reduces occurrence of background staining.
    5. Drain slides. Blot and tap excess water to prevent water spotting.
    6. Dry slides in a 60°C oven for a minimum of 30 minutes or overnight at room temperature.
    7. Store dried treated slides in a clean slide box at room temperature.
        1. Slides will adhere together if not thoroughly dried before storing.
    8. Wash emptied slide racks after use to ensure adhesive is removed.

 

PROCEDURE NOTES:

    1. Store used Aminoalkylsilane Slide Adhesive in a separate well sealed container; reuse for up to three weeks.
    2. 250 ml of Aminoalkylsilane Slide Adhesive will treat 400 slides.
    3. Aminoalkylsilane Slide Adhesive is initially clear or light amber in color and may become cloudy and turn darker in color as it ages.
        1. Adhesive effectiveness will not diminish if these changes occur.
    4. Tissue section will strongly adhere to treated slide from first contact, position section carefully.
    5. To prevent formation of water blisters trapped between paraffin section and glass, drain and dry sectioned slides in a vertical position.

 

REFERENCES:

  1. Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 70.
  2. Henderson, Colin. “Aminoalkylsilane: An Inexpensive Simple Preparation for Slide Adhesion.” The Journal of Histotechnology2 (1989): 123-124.
  3. Modifications developed by Newcomer Supply Laboratory.