Alcian Blue Stain 1%, pH 1.0 Aqueous
(use: Sulfated Acidic Mucosubstances)
|250 ml||500 ml|
|Alcian Blue Stain 1%, pH 1.0 Aqueous||Part 1005A||Part 1005B|
|Hydrochloric Acid 0.1N, Aqueous||Part 1207|
|Nuclear Fast Red Stain, Kernechtrot||Part 1255|
|Xylene, ACS||Part 1445|
|Alcohol, Ethyl Denatured, 100%||Part 10841|
|Alcohol, Ethyl Denatured, 95%||Part 10842|
For storage requirements and expiration date refer to individual bottle labels.
Newcomer Supply Alcian Blue Stain 1%, pH 1.0 Aqueous is for the specific staining of sulfated acid epithelial mucins in the Alcian Blue 1%, pH 1.0 procedure.
Fixation: Formalin 10%, Phosphate Buffered (Part 1090)
Technique: Paraffin sections cut at 4 microns
Solutions: All solutions manufactured by Newcomer Supply, Inc.
All Newcomer Supply stain procedures are designed to be used with Coplin jars filled to 40 ml following the provided staining procedure.
- If necessary, heat dry tissue sections/slides in oven.
- Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each. Wash well with distilled water.
- See procedure note #1.
- Rinse briefly in Hydrochloric Acid 0.1N, Aqueous (Part 1207).
- Save Hydrochloric Acid 0.1N, Aqueous for Step #5.
- Stain in Alcian Blue Stain 1%, pH 1.0 Aqueous for 30 minutes at room temperature.
- Rinse briefly in Hydrochloric Acid 0.1N, Aqueous.
- Do not wash in water.
- A water wash may alter pH level resulting in nonspecific staining.
- Blot slide(s) dry with filter paper.
- Counterstain dried slide(s) with Nuclear Fast Red Stain, Kernechtrot (Part 1255) for 5 minutes.
- Shake solution well before use; do not filter.
- Rinse well in distilled water.
- See Procedure Note #2.
- Dehydrate quickly in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.
|Sulfated acid epithelial mucin||Blue|
|Cytoplasm and background||Pink|
|Nuclei||Pink to red|
- Drain slides after each step to prevent solution carry over.
- Wash well after Nuclear Fast Red Stain, Kernechtrot to avoid cloudiness in dehydration steps.
- If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.
- Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009.146-147.
- Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 173.
- Modifications developed by Newcomer Supply Laboratory.