Zinc Formalin Fixative
(use: On auto processor or as a prefixative. Working solution. Replacement for Zinc Formalin Sensitizer in Steiner-Chapman Stain.)
- Shelf Life 2 years from date of manufacture.
|1 Liter||1 Gallon||20 Liter Cube|
|Zinc Formalin Fixative||Part 1482A||Part 1482B||Part 1482C|
For storage requirements and expiration date refer to individual bottle labels.
Newcomer Supply Zinc Formalin Fixative (ZFF) is a ready-to-use unbuffered zinc sulfate solution, recommended as an all-purpose tissue fixative for demonstration of crisp nuclear detail, superior cellular morphology, enhanced hematoxylin and eosin (H&E) staining, special staining and immunohistochemical (IHC) studies. This zinc sulfate fixative presents minimal safety hazards and is non-corrosive.
Zinc Formalin Fixative can also be used as a substitute for Zinc Formalin Sensitizer in the Steiner-Chapman Modified Silver Stain Kit (Part 9172).
Small biopsies: Minimum of 2-6 hours
Larger specimens: Minimum of 6-8 hours
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
Place fresh tissue specimen in Zinc Formalin Fixative as soon as possible after surgical excision.
- See Procedure Note #1.
Hold tissue in Zinc Formalin Fixative until ready to process.
- See Procedure Note #2.
Tissue Processor Fixation with Zinc Formalin Fixative:
- Refer to manufacturer specifications for any restrictions on the use of zinc sulfate fixative stations on their tissue processor instrumentation.
- A 70% or lower alcohol percentage is recommended in the processor’s first dehydration station to deter formation of zinc precipitate in tissue specimens and processor solutions.
Post-Fixation Formalin 10%, Phosphate Buffered:
- Wash Zinc Formalin Fixative fixed tissue thoroughly in distilled water for a minimum of 10 minutes to remove residual zinc and deter formation of formalin pigment.
- Place on tissue processor in Formalin 10%, Phosphate Buffered fixation step.
A specimen initially received in Formalin 10%, Phosphate Buffered should be thoroughly rinsed in tap water prior to placing in Zinc Formalin Fixative as a post-fixative.
- Failure to rinse tissue may increase the possibility of zinc precipitate formation in tissue specimens.
- Extended storage of tissue in Zinc Formalin Fixative will not affect antigenicity or excessively harden tissue.
- Discard Zinc Formalin Fixative accounting to local and state environmental regulations.
Zinc Formalin Fixative can be neutralized with magnesium hydroxide/oxide, sodium carbonate or sodium bicarbonate to precipitate zinc at pH 7.0-8.0.
- Approximately 100 grams of sodium bicarbonate will precipitate zinc from 1 liter of Zinc Formalin Fixative.
- Separate solids from liquid and dispose of according to local and state environmental regulations.
- Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 22-23, 266-267.
- Dapson, Janet Crookham, and Richard Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling, and Disposal. 4th ed. Battle Creek, MI: Anatech, 2005. 148, 279.
- L’Hoste, Robert J., and Mary Ann Tourres. “Using Zinc Formalin as a Routine Fixative in the Histology Laboratory.” Laboratory Medicine 26.3 (1995): 210-214.
- Modifications developed by Newcomer Supply Laboratory.