Slide Brite

An extremely safe, odorless alternative to xylene. Gentle on tissue and does not make it brittle. Excellent for derms and biopsy tissue. No tissue shrinkage or morphology changes. Enhances nuclear detail. Non-flammable with a flash point of 142°F. Use with S-Mount Mounting Medium for optimal results. No hazardous shipping fees!

 

CLICK HERE for information on the mounting medium that is recommended for this product

 

Product Options:

Part # ABSB-04 4 x1 gal/cs $177.00

SOLUTION:

4 X 1 Gallon
Slide Brite™ Part ABSB-04

 

Additionally Needed:

Alcohol, Ethyl Denatured, 70% Part 10844
Alcohol, Ethyl Denatured, 95% Part 10842
Alcohol, Ethyl Denatured, 100% Part 10841
S Mounting Medium Part 6750

For storage requirements and expiration date refer to individual bottle labels.

 

APPLICATION:

Newcomer Supply Slide Brite™ Xylene Substitute is classified as an aliphatic hydrocarbon that provides a safe alternative to xylene, reduces risks and improves personnel safety in the laboratory.  Benefits of Slide Brite™ Xylene Substitute include:

    • Odorless, non-hazardous, non-irritating and fast drying.
    • Gentle on tissue and enhanced nuclear detail.
    • No tissue brittleness, shrinkage or adverse morphologic changes.
    • Compatible with IHC staining.
    • Does not require hazardous/flammable storage.
    • Flash point of 61°C/142°F (29°C/84°F flash point of xylene)
    • No vapor monitoring.
    • Compatible on tissue processors and staining systems.

 

METHOD:

Fixation: Formalin 10%, Phosphate Buffered (Part 1090)

Technique:  Paraffin, frozen sections, smears

 

PROCESSING PROCEDURE: 

    1. Refer to Slide Brite™ Tissue Processing Schedule.
    2. Use three Slide Brite™ clearing stations, 60 minutes each.
        1. For two clearing stations, allow 90 minutes per station.
    3. Rotate, filter and/or replace Slide Brite™ stations daily or after processing approximately 1000 blocks.
    4. Test and optimize Slide Brite™ as a clearing agent in tissue processing schedules prior to standard use.

 

STAINING PROCEDURE:

    1. Refer to Slide Brite™ Staining Procedure.
    2. For best deparaffinization results, place warm slides directly from dryer/oven into Slide Brite™.
    3. Deparaffinize warm slides thoroughly in three changes of Slide Brite™, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
        1. See Procedure Notes #1, #2 and #3.
    4. Proceed with staining protocol.
    5. Dehydrate in two changes of 95% and three changes of 100% ethyl alcohol. Clear in four changes of Slide Brite™.
    6. Coverslip with S Mounting Medium.
        1. See Procedure Note #4.
    7. Test and optimize Slide Brite™ in staining procedures and automated staining systems prior to standard use.

 

PROCEDURE NOTES:

    1. Deparaffinization and clearing steps may require longer timings then xylene.
    2. Due to absorption rates Slide Brite™ will require more frequent changes compared to xylene.
    3. Any water contamination will sit on top of Slide Brite™.
        1. Minimize water presence by using Hydrosorb-X Water Absorbing Packets (STHXP) or decant water off.
    4. Test Slide Brite™ compatibility with other mounting mediums prior to use.
        1. If mounting medium is not readily miscible or displays separation it is incompatible with Slide Brite™,
    5. Slide Brite™ is not recommended for automated coverslippers.
    6. Slide Brite™ will not remove adhered coverslips as well as xylene.
    7. Refer to manufacturer’s specifications on the use of Slide Brite™ on all instrumentation.

 

REFERENCES:

    1. Dapson, Janet Crookham, and Richard W. Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling and Disposal. 4th Battle Creek, MI: Anatech, 2005. 150-155, 235.
    2. Wynnchuk, Maria. “Evaluation of Xylene Substitutes for Paraffin Tissue Processing.” The Journal of Histotechnology2 (1994): 143-149.
    3. Modifications developed by Newcomer Supply Laboratory.

 

Slide Brite™ Routine Tissue Processing Schedule

Station Solution/Reagent Heat Vacuum Time
1 Formalin 10%, Phosphate Buffered Off Off 90 Minutes
2 Formalin 10%, Phosphate Buffered Off Off 90 Minutes
3 70% Alcohol, Ethyl Denatured Off Off 30 Minutes
4 95% Alcohol, Ethyl Denatured Off Off 40 Minutes
5 100% Alcohol, Ethyl Denatured Off 15 mm Hg 50 Minutes
6 100% Alcohol, Ethyl Denatured Off 15 mm Hg 40 Minutes
7 100% Alcohol, Ethyl Denatured Off 15 mm Hg 40 Minutes
8 Slide Brite™ Off/On 38°C 15 mm Hg 60 Minutes
9 Slide Brite™ Off/On 38°C 15 mm Hg 60 Minutes
10 Slide Brite™ Off/On 38°C 15 mm Hg 60 Minutes
11 Paraffin 60°C 15 mm Hg 120 Minutes
12 Paraffin 60°C 15 mm Hg 60 Minutes

 

Slide Brite™ Routine Tissue Processing Notes:

    1. When using only two clearing stations, increase time from 60 minutes to 90 minutes per station (Steps #8 to #10).
    2. Rotate, filter and/or replace Slide Brite™ solutions daily or after processing approximately 1000 blocks.

 

Slide Brite™ Staining Procedure                                                                                               

Step Solution/Reagent Time
1 Slide Dryer/Oven 58°C-60°C
2 Slide Brite™ 3 Minutes
3 Slide Brite™ 3 Minutes
4 Slide Brite™ 3 Minutes
5 100% Alcohol, Ethyl Denatured 30 Seconds/10 Dips
6 100% Alcohol, Ethyl Denatured 30 Seconds/10 Dips
7 95% Alcohol, Ethyl Denatured 30 Seconds/10 Dips
8 95% Alcohol, Ethyl Denatured 30 Seconds/10 Dips
9 Distilled Water Rinse 30 Seconds Minimum
10 Proceed with Staining Protocol.
11 95% Alcohol, Ethyl Denatured 30 Seconds/10 Dips
12 95% Alcohol, Ethyl Denatured 30 Seconds/10 Dips
13 100% Alcohol, Ethyl Denatured 30 Seconds/10 Dips
14 100% Alcohol, Ethyl Denatured 1 Minute
15 100% Alcohol, Ethyl Denatured 1 Minute
16 Slide Brite™ Clearing Agent 1 Minute
17 Slide Brite™ Clearing Agent 1 Minute
18 Slide Brite™ Clearing Agent 2 Minutes
19 Slide Brite™ Clearing Agent 2 Minutes
20 Coverslip with S Mounting Medium

 

Slide Brite™ Staining Procedure Notes:

    1. For best deparaffinization results, place warm slides directly from dryer/oven into Slide Brite™ (Step #2).
    2. To achieve best coverslipping results, S Mounting Medium (Part 6750) is the recommended mounting medium with Slide Brite™.