Mold Release Concentrate
(use: Help blocks release from embedding molds.)
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- Shelf Life is 2 years from date of manufacture.
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SOLUTION:
120 ml | 120 ml X 6 | |
Mold Release Concentrate | Part 1246A | Part 1246A |
Additionally Needed:
Alcohol, Ethyl Denatured, 100% OR Alcohol, Isopropyl ACS, 100% |
Part 10841 OR Part 12094 |
Base Molds, Stainless Steel | Part 5104 |
Base Molds, Disposable | Part 5105 |
For storage requirements and expiration date refer to individual bottle labels.
APPLICATION:
Newcomer Supply Mold Release Concentrate, when diluted to a working mold release solution, facilitates the clean release of cooled, solidified paraffin embedded tissue blocks from tissue embedding base molds.
METHOD:
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
PROCEDURE:
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- Dilute Mold Release Concentrate to a working solution. Combine solutions in a plastic spray bottle and mix well;
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- Mold Release Concentrate 5 ml
- Ethyl Alcohol or Isopropyl Alcohol 95 ml
- Store unused Mold Release Working Solution at room temperature for up to 6 months.
- Keep working solution tightly capped when not in use.
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- Arrange clean and dry stainless steel base molds (Part 5104) or plastic disposable base molds (Part 5105) on paper towels or bench paper in an adequately ventilated area.
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- See Procedure Note #1.
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- Spray all embedding base molds thoroughly with Mold Release Working Solution, allowing small beads of solution to form on base mold surfaces that will come in contact with paraffin.
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- See Procedure Note #2.
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- Allow embedding base molds to thoroughly air dry prior to use.
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- Thorough drying of treated embedding base molds is necessary to avoid residual Mold Release Solution mixing with paraffin during embedding process.
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- Embed paraffin processed tissue in appropriate size embedding base molds that have been Mold Release treated and dried.
- Cool paraffin blocks on embedding center cold plate.
- After paraffin has completely solidified, cooled tissue blocks will easily separate from embedding base molds.
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- See Procedure Note #3.
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- Repeat treatment of clean and dry base molds with Mold Release Working Solution on a routine basis.
- Dilute Mold Release Concentrate to a working solution. Combine solutions in a plastic spray bottle and mix well;
PROCEDURE NOTES:
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- If plastic disposable base molds are reused, Mold Release Working Solution will assist with easier block release.
- Spraying embedding base molds provides a better adherence of Mold Release and preferred over soaking base molds in solution.
- If tissue blocks are difficult to remove from embedding base molds, a sharp tap of the base mold on the bench top will facilitate removal.
REFERENCES:
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- Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 66.
- Modifications developed by Newcomer Supply Laboratory.