Mold Release Concentrate

(use: Help blocks release from embedding molds.)

  • Shelf Life is 2 years from date of manufacture.

 

Product Options:

1 - 5:
Part # 1246A 120 ml $14.20 H
6 or more:
Part # 1246A 120 ml $11.90 H

 

 

SOLUTION:                                                                

120 ml 120 ml X 6
Mold Release Concentrate Part 1246A Part 1246A

 

Additionally Needed:

Alcohol, Ethyl Denatured, 100%

                      OR

Alcohol, Isopropyl, ACS, 100%

Part 10841

OR

Part 12094

Base Molds, Stainless Steel  Part 5104
Base Molds, Disposable Part 5105

For storage requirements and expiration date refer to individual bottle labels.

 

APPLICATION:

Newcomer Supply Mold Release Concentrate, when diluted to a working mold release solution, facilitates the clean release of cooled, solidified paraffin embedded tissue blocks from tissue embedding base molds.

 

METHOD:

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

PROCEDURE:

  1. Dilute Mold Release Concentrate to a Mold Release Working Solution following exact recommended proportions.  Combine solutions in a plastic spray bottle and mix well;
    1. Mold Release Concentrate                5 ml
    2. Ethyl Alcohol or Isopropyl Alcohol   95 ml
    3. Store unused Mold Release Working Solution at room temperature for up to 6 months.
    4. Keep working solution tightly capped when not in use.
  2. Arrange clean and dry stainless steel base molds (Part 5104) or plastic disposable base molds (Part 5105) on paper towels or laboratory bench paper in an adequately ventilated area; preferably under a fume hood.
    1. See Procedure Note #1.
  3. Spray all embedding base molds thoroughly with Mold Release Working Solution, allowing small beads of solution to form on all embedding base mold surfaces that will come in contact with paraffin.
    1. See Procedure Note #2.
  4. Allow embedding base molds to thoroughly air dry prior to use.
    1. Evaporation process will leave Mold Release adhered to embedding base molds, allowing easy removal of cooled embedded tissue blocks.
    2. Thorough drying of treated embedding base molds will alleviate problems with tissue sectioning that could occur with wet base molds and carry over of Mold Release Solution mixing with paraffin.
  5. Embed paraffin processed tissue in appropriate size embedding base molds that have been Mold Release treated and dried.
  6. Cool paraffin blocks on embedding center cold plate.
  7. After paraffin has completely solidified, cooled embedded tissue blocks will easily and completely separate from embedding base molds.
    1. See Procedure Note #3.
  8. Repeat treatment of clean and dry base molds with Mold Release Working Solution on a routine basis according to laboratory protocol.

 

PROCEDURE NOTES:

  1. If plastic disposable base molds are reused multiple times, the use of Mold Release Working Solution will assist with easier block release.
  2. Spraying embedding base molds provides a better adherence of Mold Release and is preferred over soaking base molds in the solution.
  3. On occasion when embedded tissue blocks may be difficult to remove from embedding base molds, a sharp tap of the embedding base mold on the bench top will facilitate removal.

 

REFERENCES:

  1. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 66.
  2. Modifications developed by Newcomer Supply Laboratory.