Haupt Gelatin Adhesive

SOLUTION:

For storage requirements and expiration date refer to individual bottle label.

APPLICATION:

Newcomer Supply Haupt Gelatin Adhesive is a blended solution of high-quality gelatin, glycerin and phenol with a wide variety of procedural uses including subbed slide/direct slide coating applications.  Haupt Gelatin Adhesive works to create a strong adhesive bond between tissue sections and microscopic slides to prevent or reduce the loss of sections due to the nature of the tissue and tissue treatments, such as:

• • Thick sections
  • Harsh staining procedures
  • Animal tissues
  • Plant preparations
  • Bone specimens
  • Resin, plastic/methyl methacrylate (MMA) sections

METHOD:

Technique:  Frozen, paraffin or resin, plastic/MMA sections
Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

PROCEDURES:

Haupt Gelatin Adhesive Subbed Slide Preparation:

1. 1. Use only clean and dry microscopic slides.
   2. Place a large drop of Haupt Gelatin Adhesive on slides, spread evenly over surface creating a thin film.
      1. 1. Allow a minimum of 30 minutes drying time.
         2. Background staining may occur with thicker films.
         3. See Procedure Note #1.

Water Bath Method for Sections on Subbed Slides:

1. 3. Fill water bath with distilled water; maintain temperature at 5°- 10°C below melting point of embedding medium.
      1. 1. See Procedure Note #2.
   4. Float paraffin tissue sections onto Haupt subbed slides.
   5. Warm slide slightly on top edge of water bath to straighten section.
   6. Drain and dry slides.

Vapor Fixation Method for Sections on Subbed Slides:

1. 7. Mount paraffin or frozen sections on Haupt subbed slides; dry 1 minute.
   8. Under fume hood, add 2-4 ml of concentrated formaldehyde to bottom of a Coplin jar.
   9. Place slides in Coplin jar; formaldehyde should not be in direct contact with tissue sections.
   10. Tightly cover and place in 60°C oven for 30 minutes to 1 hour.
       1. 1. See Procedure Note #3.
   11. Remove slides from Coplin jar; drain and dry.

Method for Resin, Plastic/MMA Sections on Subbed Slides:

1. 12. Place droplets of filtered/processed water on Haupt subbed slides.
   13. Transfer resin or plastic/MMA sections to water droplets.
   14. Air dry or use low heat.

Method for Resin, Plastic/MMA Sections on Non-Subbed Slides:

1. 15. Place droplets of Haupt Gelatin Adhesive on clean, dry slides.
   16. Transfer resin or plastic/MMA sections directly to Haupt droplets.
   17. Add 1-2 drops of 50% ethanol atop sections.
   18. Manipulate sections by teasing or stretching to remove any folds.
   19. Air dry or use low heat.

PROCEDURE NOTES:

1. 1. Dry slides in dust-free environment and store dried subbed slides indefinitely in a slide box at room temperature and low humidity.
   2. Clean interior/exterior of water bath on a daily basis to deter contaminates and possibility of residual gelatin adhesive build-up.
   3. Formalin vapor renders gelatin insoluble, strongly affixing tissue sections to subbed slides.

REFERENCES:

1. 1. Haupt, Arthur W. “A Gelatin Fixative for Paraffin Sections.” Stain Technology3 (1930): 97-98.
   2. Huang, Bing Quan., Michael John. Sumner, Claudio Stasolla, and Edward C. Yeung. Plant Microtechniques and Protocols. Springer, 2015. 88, 94.
   3. Luna, Lee G. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Gaitheresburg, MD: American Histolabs, 1992. 581.
   4. Presnell, Janice, Martin Schreibman, and Gretchen Humason. Humason’s Animal Tissue Techniques. 5th ed. Baltimore: Johns Hopkins University Press, 1997. 468.
   5. Modifications developed by Newcomer Supply Laboratory.