Davidson Fixative
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- Shelf Life is 2 years from date of manufacture.
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SOLUTION:
1 Gallon | |
Davidson Fixative | Part 1045A |
For storage requirements and expiration date refer to individual bottle label.
APPLICATION:
Newcomer Supply Davidson Fixative is an alcohol-formalin-acetic acid based fixative with human, veterinary and research applications. This ready-to-use fixative (also known as Hartmann’s Solution) is recommended for a variety of specimens, including eyes and testes. Davidson Fixative penetrates structures quickly, while preserving morphological detail and immunohistochemical (IHC) staining.
Tissues placed in Davidson Fixative turn white/opaque, enhancing the visibility and yield of lymph nodes in fatty breast, colon and radical dissections. Overnight fixation is recommended for large and/or fatty specimens and lymph node detection.
METHOD:
Fixation Recommendations:
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- Small Biopsies: Up to 24 hours.
- Mice Eyes: Up to 12 hours.
- Rat and Rabbit Eyes: Up to 24 hours.
- Large Eyes (human or animal): 48-72 hours.
- Mollusks: 24-48 hours.
- Lymph Nodes: Up to 24 hours.
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- Small nodes (5 mm or less) should be halved.
- Dissect larger nodes with no piece thicker than 2-3 mm.
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Solutions: All solutions are manufactured by Newcomer Supply, Inc.
FIXATION PROCEDURE:
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- Place tissue directly in Davidson Fixative after surgical excision.
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- See Procedure Note #1.
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- Fix in Davidson Fixative for the recommended fixation time.
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- See Procedure Note #2.
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- Rinse Davidson fixed tissue in distilled water; 1-2 minutes.
- Hold tissue in either Formalin 10%, Phosphate Buffered (Part 1090) or in 70% Ethyl Alcohol (Part 10844) prior to processing.
- Place tissue directly in Davidson Fixative after surgical excision.
PROCEDURE NOTES:
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- If received in Formalin 10%, Phosphate Buffered, rinse tissue thoroughly in tap water prior to placing in Davidson Fixative.
- Extended storage in Davidson Fixative is not recommended and may result in hard, brittle tissue.
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- After maximum fixation, transfer Davidson fixed tissue to 70% Ethyl Alcohol or Formalin 10%, Phosphate Buffered for long-term storage purposes.
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REFERENCES:
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- Eltoum, Isam, Jerry Fredenburgh, Russell Myers and William Grizzle. “Introduction to the Theory and Practice of Fixation of Tissues.” The Journal of Histotechnology 24.3 (2001): 173-190.
- Howard, Dorothy, Earl Lewis, Jane Keller and Cecilia Smith. Histological Techniques for Marine Bivalve Mollusks and Crustaceans. 2nd ed. Oxford, MD: NOAA, National Ocean Service, 2004. 60.
- Kiernan, J. A. Histological and Histochemical Methods: Theory and Practice. 3rd ed. London, Ontario: Arnold, 2003. 28-29.
- Latendresse, John R., Alan R. Warbrittion, Henning Jonassen and Dianne M. Creasy. “Fixation of Testes and Eyes Using a Modified Davidson’s Fluid: Comparison with Bouin’s Fluid and Conventional Davidson’s Fluid.” Toxicologic Pathology (2002): 524-33.
- Modifications developed by Newcomer Supply Laboratory.