Products

Carson Modified Millonig Formalin

Newcomer Supply Beaker

(use: Light & Electron Microscopy)

      • Shelf Life is 2 years from date of manufacture.

Product Options:

Part # 12445A 1 gal $15.00
Part # 12445C 20 L cube $52.20

SOLUTION:

1 Gallon 20 Liter Cube
Carson Modified Millonig Formalin Part 12445A Part 12445C

 

30 ml vial, 15 ml fill (100/cs)
Carson Modified Millonig Formalin Vial Part 12445E

 

For storage requirements and expiration date refer to individual bottle labels.

 

APPLICATION:

Newcomer Supply Carson Modified Millonig Formalin, is a formalin based ready-to-use fixative buffered with sodium monobasic phosphate and sodium hydroxide.  Carson Modified Millonig Formalin promotes fixation with rapid penetration, providing excellent cellular detail and ultrastructural preservation.

This multi-purpose fixative has applications for both light microscopy (LM) and electron microscopy (EM) studies, including immunogold labeling, and can be used in place of standard 10% formalin fixatives.

Carson Modified Millonig Formalin Vials are available in 30 ml vials prefilled with 15 ml of fixative for ease of use, storage and transport.

 

METHOD:

Fixation Recommendations:

    • Small Biopsies: Minimum of 1-2 hours.
    • Larger Specimens: Minimum of 4-6 hours.

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

FIXATION PROCEDURE:

    1. Place fresh tissue in Carson Modified Millonig Formalin after surgical excision.
        1. See Procedure Notes #1 and #2.
    2. Hold tissue in Carson Modified Millonig Formalin for processing.
        1. See Procedure Note #3.
    3. Processing:
        1. Light Microscopy: place on tissue processor starting in primary fixation step or first dehydration station.
        2. Electron Microscopy: A secondary osmium tetroxide fixation is recommended. Refer to protocol for electron microscopy processing.

 

PROCEDURE NOTES:

    1. If received in Formalin 10%, Phosphate Buffered (Part 1090), rinse tissue thoroughly in tap water prior to placing in Carson Modified Millonig Formalin.
    2. For electron microscopy studies, fix tissue within 15 minutes after excision. Mince into 1 mm cubes for expedient fixative infiltration.
    3. Tissue can remain in Carson Modified Millonig Formalin over an extended period of time.

 

REFERENCES:

    1. Bancroft, John D. and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 68.
    2. Carson, Freida L. and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 11-12, 335-336.
    3. Carson, Freida L. and James Martin. “Formalin Fixation for Electron Microscopy.” The Journal of Histotechnology 2.2 (1979): 58-60.
    4. Sheehan, Dezna C. and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 46.
    5. Modifications developed by Newcomer Supply Laboratory.