Zinc Formalin Fixative

(use: On auto processor or as a prefixative. Working solution. Replacement for Zinc Formalin Sensitizer in Steiner-Chapman Stain.)

  • Shelf Life 2 years from date of manufacture.

 

Product Options:

Part # 1482A1 L $13.50
Part # 1482B1 gal $25.00
Part # 1482C20 L $88.80

SOLUTION:                                                                                                                                     

  1 Liter 1 Gallon 20 Liter Cube
Zinc Formalin Fixative Part 1482A Part 1482B Part 1482C

               

For storage requirements and expiration date refer to individual bottle labels.

 

APPLICATION:

Newcomer Supply Zinc Formalin Fixative (ZFF) is a ready-to-use unbuffered zinc sulfate solution, recommended as an all-purpose tissue fixative for demonstration of crisp nuclear detail, superior cellular morphology, enhanced hematoxylin and eosin (H&E) staining, special staining and immunohistochemical (IHC) studies.  This zinc sulfate fixative presents minimal safety hazards and is non-corrosive.

Zinc Formalin Fixative can also be used as a substitute for Zinc Formalin Sensitizer in the Steiner-Chapman Modified Silver Stain Kit (Part 9172).

 

METHOD:

Fixation:

Small biopsies:  Minimum of 2-6 hours

Larger specimens: Minimum of 6-8 hours

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

FIXATION PROCEDURE:

  1. Place fresh tissue specimen in Zinc Formalin Fixative as soon as possible after surgical excision.
    1. See Procedure Note #1.
  2. Hold tissue in Zinc Formalin Fixative until ready to process.
    1. See Procedure Note #2.
  3. Tissue Processor Fixation with Zinc Formalin Fixative:
    1. Refer to manufacturer specifications for any restrictions on the use of zinc sulfate fixative stations on their tissue processor instrumentation.
    2. A 70% or lower alcohol percentage is recommended in the processor’s first dehydration station to deter formation of zinc precipitate in tissue specimens and processor solutions.
  4. Post-Fixation Formalin 10%, Phosphate Buffered:
    1. Wash Zinc Formalin Fixative fixed tissue thoroughly in distilled water for a minimum of 10 minutes to remove residual zinc and deter formation of formalin pigment.
    2. Place on tissue processor in Formalin 10%, Phosphate Buffered fixation step.

 

PROCEDURE NOTES:

  1. A specimen initially received in Formalin 10%, Phosphate Buffered should be thoroughly rinsed in tap water prior to placing in Zinc Formalin Fixative as a post-fixative.
    1. Failure to rinse tissue may increase the possibility of zinc precipitate formation in tissue specimens. 
  2. Extended storage of tissue in Zinc Formalin Fixative will not affect antigenicity or excessively harden tissue.
  3. Discard Zinc Formalin Fixative accounting to local and state environmental regulations.
  4. Zinc Formalin Fixative can be neutralized with magnesium hydroxide/oxide, sodium carbonate or sodium bicarbonate to precipitate zinc at pH 7.0-8.0.
    1. Approximately 100 grams of sodium bicarbonate will precipitate zinc from 1 liter of Zinc Formalin Fixative.
    2. Separate solids from liquid and dispose of according to local and state environmental regulations.

 

REFERENCES:

  1. Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 22-23, 266-267.
  2. Dapson, Janet Crookham, and Richard Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling, and Disposal. 4th ed. Battle Creek, MI: Anatech, 2005. 148, 279.
  3. L’Hoste, Robert J., and Mary Ann Tourres. “Using Zinc Formalin as a Routine Fixative in the Histology Laboratory.” Laboratory Medicine 26.3 (1995): 210-214.
  4. Modifications developed by Newcomer Supply Laboratory.