Hollande Fixative
(use: Recommended for GI Biopsies.)
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- Shelf Life is 2 years from date of manufacture.
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SOLUTION:
1 Liter | 1 Gallon | |
Hollande Fixative | Part 1208A | Part 1208B |
For storage requirements and expiration date refer to individual bottle labels.
APPLICATION:
Newcomer Supply Hollande Fixative, a ready-to-use picric acid based fixative with a copper acetate component that provides excellent morphology preservation, increased nuclear and sub-cellular detail while stabilizing red blood cell membranes. Hollande Fixative is recommended for gastrointestinal tract biopsies and endocrine tissue.
METHOD:
Fixation Recommendations:
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- Small Biopsies: A minimum of 1 hour.
- Larger Biopsies: 4 hours to 24 hours.
- To facilitate grossing, place in Hollande Fixative for 1 hour to firm; trim to smaller pieces.
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
FIXATION PROCEDURE:
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- Place fresh tissue in Hollande Fixative after surgical excision.
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- See Procedure Notes #1 and #2.
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- The acidic nature of Hollande Fixative is generally enough to decalcify small bone without additional decalcification steps.
- Rinse Hollande fixed tissue thoroughly in running tap water for a minimum of 15 minutes, followed by a minimum of 15 minutes in 70% ethyl alcohol (10844) prior to processing.
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- See Procedure Notes #3 and #4.
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- Place on tissue processor starting in Formalin 10%, Phosphate Buffered (Part 1090) fixation step.
- Place fresh tissue in Hollande Fixative after surgical excision.
PROCEDURE NOTES:
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- If received in Formalin 10%, Phosphate Buffered, rinse tissue thoroughly in tap water prior to placing in Hollande Fixative.
- Hollande Fixative may grossly stain tissue green. This is normal.
- It is essential to thoroughly wash Hollande fixed tissue prior to tissue processing or an insoluble blue phosphate precipitate will form upon exposure to Formalin 10%, Phosphate Buffered.
- Extended storage in Hollande Fixative is not recommended.
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- After maximum fixation, wash tissue in running tap water for a minimum of 15 minutes, then a minimum of 15 minutes in 70% ethyl alcohol.
- Transfer Hollande fixed tissue to Formalin 10%, Phosphate Buffered for long-term storage purposes.
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- Dispose of Hollande Fixative as picric acid hazardous waste according to local/state regulations.
REFERENCES:
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- Bancroft, John D. and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 70.
- Carson, Freida L. and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 21.
- Dapson, Janet Crookham and Richard Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling, and Disposal. 4th ed. Battle Creek, MI: Anatech, 2005. 150, 265-266.
- Goss, Gwen. “Hollande’s Solution as a Routine Tissue Fixative.” Lecture, Region IV Meeting, National Society for Histotechnology, Chicago, June 1990.
- Hollande, A. Ch. “Enrichment of Bouin’s Liquid Picric Acid by the Addition of Neutral Copper Acetate.” Report of the Society of Biology 81 (1918): 17.
- Modifications developed by Newcomer Supply Laboratory.