Products

Hollande Fixative

Newcomer Supply Beaker

(use: Recommended for GI Biopsies.)

      • Shelf Life is 2 years from date of manufacture.

Product Options:

Part # 1208A 1 L $70.00
Part # 1208B 1 gal $143.00

SOLUTION:

1 Liter 1 Gallon
Hollande Fixative Part 1208A Part 1208B

 

For storage requirements and expiration date refer to individual bottle labels.

 

APPLICATION:

Newcomer Supply Hollande Fixative, a ready-to-use picric acid based fixative with a copper acetate component that provides excellent morphology preservation, increased nuclear and sub-cellular detail while stabilizing red blood cell membranes. Hollande Fixative is recommended for gastrointestinal tract biopsies and endocrine tissue.

 

METHOD:

Fixation Recommendations:

    • Small Biopsies: A minimum of 1 hour.
    • Larger Biopsies: 4 hours to 24 hours.
    • To facilitate grossing, place in Hollande Fixative for 1 hour to firm; trim to smaller pieces.

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

FIXATION PROCEDURE:

    1. Place fresh tissue in Hollande Fixative after surgical excision.
        1. See Procedure Notes #1 and #2.
    2. The acidic nature of Hollande Fixative is generally enough to decalcify small bone without additional decalcification steps.
    3. Rinse Hollande fixed tissue thoroughly in running tap water for a minimum of 15 minutes, followed by a minimum of 15 minutes in 70% ethyl alcohol (10844) prior to processing.
        1. See Procedure Notes #3 and #4.
    4. Place on tissue processor starting in Formalin 10%, Phosphate Buffered (Part 1090) fixation step.

 

PROCEDURE NOTES:

    1. If received in Formalin 10%, Phosphate Buffered, rinse tissue thoroughly in tap water prior to placing in Hollande Fixative.
    2. Hollande Fixative may grossly stain tissue green. This is normal.
    3. It is essential to thoroughly wash Hollande fixed tissue prior to tissue processing or an insoluble blue phosphate precipitate will form upon exposure to Formalin 10%, Phosphate Buffered.
    4. Extended storage in Hollande Fixative is not recommended.
        1. After maximum fixation, wash tissue in running tap water for a minimum of 15 minutes, then a minimum of 15 minutes in 70% ethyl alcohol.
        2. Transfer Hollande fixed tissue to Formalin 10%, Phosphate Buffered for long-term storage purposes.
    5. Dispose of Hollande Fixative as picric acid hazardous waste according to local/state regulations.

 

REFERENCES:

    1. Bancroft, John D. and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 70.
    2. Carson, Freida L. and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 21.
    3. Dapson, Janet Crookham and Richard Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling, and Disposal. 4th ed. Battle Creek, MI: Anatech, 2005. 150, 265-266.
    4. Goss, Gwen. “Hollande’s Solution as a Routine Tissue Fixative.” Lecture, Region IV Meeting, National Society for Histotechnology, Chicago, June 1990.
    5. Hollande, A. Ch. “Enrichment of Bouin’s Liquid Picric Acid by the Addition of Neutral Copper Acetate.” Report of the Society of Biology 81 (1918): 17.
    6. Modifications developed by Newcomer Supply Laboratory.