(use: Recommended for GI Biopsies.)
- Shelf Life is 2 years from date of manufacture.
|SOLUTION:||1 Liter||1 Gallon|
|Hollande Fixative||Part 1208A||Part 1208B|
For storage requirements and expiration date refer to individual bottle labels.
Newcomer Supply Hollande Fixative, a ready-to-use picric acid based fixative with an added copper acetate component, provides excellent morphology preservation, increased nuclear and sub-cellular detail while stabilizing red blood cell membranes. Hollande Fixative is recommended for gastrointestinal tract biopsies and endocrine tissue.
- Larger Biopsies: A minimum of 4 hours to a maximum of 24 hours. To facilitate grossing, the tissue can be placed in Hollande Fixative for an hour to firm tissue; then cleanly trim to smaller pieces.
- Small Biopsies: A minimum of 1 hour is recommended.
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
Place fresh tissue specimen in Hollande Fixative as soon as possible after surgical excision.
- See Procedure Notes #1 and #2.
Hold tissue specimens in Hollande Fixative until ready to process or a maximum of 24 hours.
- See Procedure Note #3.
Rinse Hollande fixed tissue thoroughly in running tap water for a minimum of 15 minutes, followed by a minimum of 15 minutes in 70% ethyl alcohol (Part 10844) prior to processing.
- See Procedure Note #4.
- Place on tissue processor starting in Formalin 10%, Phosphate Buffered (Part 1090) fixation step.
- The acidic nature of Hollande Fixative is generally enough to sufficiently decalcify small bone without additional decalcification steps.
- Hollande Fixative may grossly stain tissue green. This reaction is normal.
- Extended storage of tissue in Hollande Fixative is not recommended. After a maximum fixation time of 24 hours wash tissue in running tap water for a minimum of 15 minutes, then a minimum of 15 minutes in 70% ethyl alcohol; transfer Hollande fixed wet tissue to Formalin 10%, Phosphate Buffered for long-term storage purposes.
- It is essential that Hollande fixed tissue is thoroughly washed prior to tissue processing or an insoluble blue phosphate precipitate will form upon exposure to Formalin 10%, Phosphate Buffered.
- Dispose of Hollande Fixative as hazardous waste and/or according to local and state environmental regulations.
- Bancroft, John D., and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 70.
- Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 21.
- Dapson, Janet Crookham, and Richard Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling, and Disposal. 4th ed. Battle Creek, MI: Anatech, 2005. 150, 265-266.
- Goss, Gwen. "Hollande's Solution as a Routine Tissue Fixative." Lecture, Region IV Meeting, National Society for Histotechnology, Chicago, June 1990.
- Hollande, A. Ch. "Enrichment of Bouin's Liquid Picric Acid by the Addition of Neutral Copper Acetate." Report of the Society of Biology 81 (1918): 17.
- Modifications developed by Newcomer Supply Laboratory.