Products

Amyloid, Animal

Amyloid, Animal Stained Histology Slide

Validation Stain: Bennhold Congo Red
Other Applicable Stains: Puchtler Congo Red, Crystal Violet-Lieb and Thioflavin 

    • Due to the nature of the tissue the standard drying method for Amyloid Control Slides are oven dried
    • Tissue sections are cut at 8 microns

Tissue: 
(+) Animal Organ
Adhesive or Charged Slides: 
Superfrost +

Product Options:

Part # 4031A 10/set $60.80
Part # 4031B 98/set $452.40

PRODUCT SPECIFICATIONS:

Tissue:  Positive staining animal organ.
Fixation: Formalin 10%, Phosphate Buffered (Part 1090).
Section/Glass: Paraffin sections cut at 8 microns on Superfrost™ Plus slides.
Quality Control Stain:  Bennhold Congo Red quality control stained slide(s) included.
Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity. 
Storage: 15-30°C in a light deprived and humidity controlled environment.
Intended Use: To verify histological techniques and reagent reactivity.

Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.

 

CONTROL SLIDE VALIDATION:

With Amyloid, Bennhold Congo Red Stain Kit: Part 9103A Individual Stain Solution
Solution A: Congo Red Stain 1%, Aqueous 250 ml Part 1038
Solution B: Alkaline Alcohol 250 ml Part 1038
Solution C: Hematoxylin Stain, Mayer Modified 250 ml Part 1202

   

APPLICATION:

Newcomer Supply Amyloid, Animal Control Slides are for the positive histochemical staining of extraneous protein deposits in amyloidosis.

 

NEWCOMER SUPPLY VALIDATION PROCEDURE:

    1. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
        1. See Procedure Notes #1 and #2.
    2. Place slides in Solution A: Congo Red Stain 1%, Aqueous; 1 hour.

        Microwave Modification: See Procedure Note #3.

        1. Place slides in a plastic Coplin jar (Part 5184) containing Solution A: Congo Red Stain 1%, Aqueous. Microwave for 3 minutes at 70°
    1. Rinse in two to three changes of tap water; rinse in distilled water.
    2. Differentiate in Solution B: Alkaline Alcohol, 5 to 30 seconds, agitating constantly until slide background is cleared of Solution A: Congo Red Stain 1%, Aqueous.
    3. Rinse in two to three changes of tap water; rinse in distilled water.
    4. Counterstain with Solution C: Hematoxylin Stain, Mayer Modified, 3 to 5 minutes.
    5. Wash in running tap water for 5 to 10 minutes.
    6. Dehydrate in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.

 

RESULTS:

Light Field Microscopy:
Amyloid Pink to red
Nuclei Blue
Polarized Light:
Amyloid fluorescence Apple green

 

PROCEDURE NOTES:

    1. Drain slides after each step to prevent solution carry over.
    2. Do not allow sections to dry out at any point during procedure.
    3. The microwave procedure was tested using a laboratory-grade microwave oven. This procedure is a guideline and techniques should be developed for use in your laboratory.
    4. Sections are cut at 8 microns to provide more intense staining and allow smaller amyloid deposits to be identified.
    5. If using a xylene substitute, follow manufacturer’s recommendation for deparaffinization and clearing steps.

 

REFERENCES:

    1. Luna, Lee G. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Gaitheresburg, MD: American Histolabs, 1992. 366-367.
    2. Sheehan, Dezna C. and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 177-178.
    3. Modifications developed by Newcomer Supply Laboratory.