Products

Alcian Blue pH 2.5, Multi-Tissue

Alcian Blue pH 2.5, Multi-Tissue Stained Histology Slide

Validation Stain: Alcian Blue 1%, pH 2.5

 

Tissue: 
(+) Umbilical Cord / (+) Small Intestine
Adhesive or Charged Slides: 
Superfrost +

Product Options:

Part # 4021A 10/set $81.80
Part # 4021B 98/set $595.00

PRODUCT SPECIFICATIONS:

Tissue:  Positive staining umbilical cord and positive staining small intestine.
Fixation: Formalin 10%, Phosphate Buffered (Part 1090).
Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.
Quality Control Stain:  Alcian Blue pH 2.5 quality control stained slide(s) included.
Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity. 
Storage: 15-30°C in a light deprived and humidity controlled environment.
Intended Use: To verify histological techniques and reagent reactivity.

Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.

 

CONTROL SLIDE VALIDATION:

With Alcian Blue 1%, pH 2.5 Stain Kit: Part 9102A/B Individual Stain Solution
Solution A: Acetic Acid 3%, Aqueous 250/500 ml Part 10017
Solution B: Alcian Blue Stain 1%, pH 2.5 Aqueous 250/500 ml Part 1003
Solution C: Nuclear Fast Red Stain, Kernechtrot 250/500 ml Part 1255

 

APPLICATION:

Newcomer Supply Alcian Blue pH 2.5, Multi-Tissue Control Slides use a combination of tissue sources for the positive histochemical staining of acid epithelial mucins (sialomucin, sulfomucin) as well as stromal (mesenchymal) mucin.

 

NEWCOMER SUPPLY VALIDATION PROCEDURE:

    1. Heat dry sections in oven according to your laboratory protocol.
    2. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
        1. See Procedure Notes #1 and #2.
    3. Place slides in Solution A: Acetic Acid 3%, Aqueous for 3 minutes.
    4. Move slides directly into Solution B: Alcian Blue Stain 1%, pH 2.5 Aqueous. Stain for 30 minutes at room temperature or for 15 minutes in a 37°C water bath.
        1. See Procedure Note #3.
    5. Wash in running tap water for 10 minutes; rinse in distilled water.
    6. Counterstain in Solution C: Nuclear Fast Red Stain, Kernechtrot for 5 minutes.
        1. Shake solution well before use; do not filter.
    7. Rinse well in distilled water.
        1. See Procedure Note #4.
    8. Dehydrate quickly through two changes of 95% ethyl alcohol and two changes of 100% ethyl alcohol. Clear in three xylene changes, 10 dips each; coverslip with compatible mounting medium.

 

RESULTS:

Acid epithelial mucins Blue
Stromal (mesenchymal) mucin Blue
Nuclei Pink-red
Cytoplasm Pale pink

 

PROCEDURE NOTES:

    1. Drain slides after each step to prevent solution carry over.
    2. Do not allow sections to dry out at any point during procedure.
    3. A brief dip in Solution A: Acetic Acid 3%, Aqueous from Step #3 can be added before Step #5 to remove excess Alcian Blue Stain.
    4. Wash well after Nuclear Fast Red Stain, Kernechtrot to avoid cloudiness in dehydration steps.
    5. If using a xylene substitute, follow manufacturer’s recommendation for deparaffinization and clearing steps.

 

REFERENCES:

      1. Carson, Freida L. and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 145-148.
      2. Sheehan, Dezna C. and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 172-175.
      3. Modifications developed by Newcomer Supply Laboratory.