Movat-Russell Pentachrome

Controls Slides Histopathology Movat-Russell Pentachrome

Validation Stain: Movat-Russell Modified Pentachrome

 

Tissue: 
(+) Small Intestine (+) Lung
Adhesive or Charged Slides: 
Superfrost+

Product Options:

Part # 4440A10/set $46.00
Part # 4440B98/set $330.00

 

 

Movat-Russell Pentachrome Control Slides contain sections of positive staining small intestine and positive staining lung.

 

PRODUCT DESCRIPTION:

The enclosed positive control slides are intended to be used to verify histological techniques and reagent reactivity.  These slides are to be used for the qualitative purpose of determining positive or negative results, and are not intended to be used for any quantitative purpose.  The first serial section within the control box is stained and provided for your reference.  Before using the unstained slides, review the enclosed stained slide with your pathologist to ensure that this tissue source is acceptable.  Newcomer Supply will not accept a return with missing slides in the series. Newcomer Supply guarantees reactivity of these control slides for one year from the date of receipt.  Revalidate after one year to verify continued reactivity.  Store at 15-30°C in a light deprived and humidity controlled environment.

These positive control slides were produced from human surgical or autopsy tissues under carefully controlled conditions.  Reasonable measures are used to deliver quality control slides that are as consistent as possible.  However, characteristics of quality control slides may be dissimilar due to variations in the reagents, stains, techniques, laboratory conditions, and tissue sources used. Newcomer Supply Laboratory uses a manual method of performing quality control procedures, specifically avoiding automation, in order to provide reactive control slides for even less aggressive methods of staining that our customers may be using.

 

CONTROL SLIDE VALIDATION:

With Movat-Russell Modified Pentachrome Stain Kit: Part 9150A Individual Stain Solution
Solution A: Alcian Blue Stain 1%, Aqueous 250 ml  
Solution B: Ammonium Hydroxide 28-30%, ACS 50 ml Part 1006
Solution C: Hematoxylin 10%, Alcoholic 100 ml  
Solution D: Ferric Chloride 10%, Aqueous 100 ml Part 10856
Solution E: Iodine, Verhoeff, Aqueous 100 ml Part 1209
Solution F: Ferric Chloride 2%, Aqueous 250 ml Part 108553
Solution G: Sodium Thiosulfate 5%, Aqueous 250 ml Part 1389
Solution H: Crocein Scarlet 7B Stain, Aqueous 250 ml  
Solution I: Acid Fuchsin Stain, Aqueous 100 ml  
Solution J: Phosphotungstic Acid 5%, Aqueous 500 ml Part 13345
Solution K: Orange G Stain 1%, Aqueous 250 ml  
Solution L: Acetic Acid 0.5%, Aqueous 500 ml Part 100121

For storage requirements and expiration date refer to individual product labels.  

 

APPLICATION:

Newcomer Supply Movat-Russell Pentachrome Control Slides are for the positive histochemical staining of connective tissue elements, mucin, fibrin, elastic fibers, muscle, and collagen.

 

METHOD:

Fixation: Formalin 10%, Phosphate Buffered (Part 1090)

Technique:  Paraffin sections cut at 5 microns on Superfrost® Plus

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

NEWCOMER SUPPLY VALIDATION PROCEDURE:

  1. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each.  Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
    1.   See Procedure Notes #1 and #2.
  2. Stain in Solution A: Alcian Blue Stain 1%, Aqueous for 20 minutes.
  3. Wash in running tap water for 5 minutes.
  4. Prepare fresh Alkaline Alcohol Solution; combine and mix well.
    1.   Solution B: Ammonium Hydroxide 28-30%             5 ml
    2.   Alcohol, Ethyl Denatured, 95% (Part 10842)               45 ml
  5. Place slides in fresh Alkaline Alcohol Solution for 30 minutes.
  6. Wash in running tap water for 10 minutes followed by a distilled water rinse.
    1.   See Procedure Note #3.
  7. Prepare fresh Hematoxylin Working Stain Solution just before use in the order given; combine and mix well.
    1.   Solution C: Hematoxylin 10%, Alcoholic                 10 ml
    2.   Alcohol, Ethyl Denatured 100% (Part 10841)                10 ml
    3.   Solution D: Ferric Chloride 10%, Aqueous             10 ml
    4.   Solution E: Iodine, Verhoeff, Aqueous                    10 ml
  1. Stain in fresh Hematoxylin Working Stain Solution for 15 minutes.
    1.   Discard after successful differentiation in Step #10.
  2. Rinse in several changes of distilled water.
  3. Differentiate one slide at a time in Solution F: Ferric Chloride 2%, Aqueous until elastic fibers contrast sharply with the background; approximately 5-10 dips.
    1.   See Procedure Note #4.
  4. Rinse in distilled water.
  5. Place in Solution G: Sodium Thiosulfate 5%, Aqueous for 1 minute.
  6. Wash in running tap water for 5 minutes; rinse in distilled water.
  7. Prepare Crocein Scarlet-Acid Fuchsin Solution:
    1.   Solution H: Crocein Scarlet 7B Stain, Aqueous                      40 ml
    2.   Solution I: Acid Fuchsin Stain, Aqueous                 10 ml
  8. Stain in Crocein Scarlet-Acid Fuchsin Solution for 1 minute.
  9. Rinse in several changes of distilled water.
  10. Rinse in Solution L: Acetic Acid 0.5%, Aqueous for 30 seconds.
  11. Place slides in Solution J:  Phosphotungstic Acid 5%, Aqueous; two changes of 5 minutes each.
  12. Rinse in Solution L: Acetic Acid 0.5%, Aqueous.
  13. Stain in Solution K: Orange G Stain 1%, Aqueous for 15 minutes. 
  14. Dehydrate through three changes of 100% ethyl alcohol, 10 dips each.   Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.
    1. Do not use 95% ethyl alcohol in the dehydration step.

 

RESULTS:

Nuclei and elastic fibers Black
Collagen and reticular fibers Yellow
Ground substance and mucin  Blue
Fibrinoid, fibrin Intense red
Muscle Red

 

PROCEDURE NOTES:

  1. Drain staining rack/slides after each step to prevent solution carry over.
  2. Do not allow sections to dry out at any point during staining procedure.
  3. It is important to completely remove Alkaline Alcohol Solution with running tap water.  Failure to do so will inhibit the subsequent staining steps.
  4. Do not over-differentiate in Solution F: Ferric Chloride 2%, Aqueous.  If the background is completely colorless, the section may be over-differentiated. Over-differentiated sections may be restained in Hematoxylin Working Stain Solution (Step #8) provided sections have not been treated with an alcohol/dehydration step.
  5. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.

 

REFERENCES:

  1. Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 172-174.
  2. Movat, Henry, “Demonstration of All Connective Tissue Elements in a Single Section”. AMA Archives of Pathology. 1955; 60 (3): 289–295.
  3. Russell H. K. Jr. “A Modification of Movat’s Pentachrome Stain”. AMA Archives of Pathology.1972; 94 (2): 187–191.
  4. Modifications developed by Newcomer Supply Laboratory.