Newcomer Supply Calibration Buffers, pH 4.01, 7.00 and 10.00 are ready-to-use buffer solutions for pH meter calibrations. Newcomer Supply standardized calibration buffers resist changes in pH and are color coded for ease of use.
- Part 1026: Calibration Buffer, pH 4.01 is a red color-coded solution for pH measurement of acidic samples.
- Part 1027: Calibration Buffer, pH 7.00 is a yellow color-coded solution for pH measurement of neutral samples.
- Part 1028: Calibration Buffer, pH 10.00 is a blue color-coded solution for pH measurement of basic samples.
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
Storage: Store all calibration buffers out of direct sunlight.
- Refer to pH meter/electrode manufacturer’s procedure manual(s) for detailed operational and maintenance instructions.
- Calibration buffers and test sample(s) should be at equal temperature prior to testing.
- Select pH buffers that frame the pH of the sample(s).
- Use pH 7.00 buffer plus at least one other pH value close to the expected measurement range.
- Pour fresh calibration buffers into individually marked beakers.
- Do not perform calibration readings directly from original buffer container.
- Close buffer containers to avoid carbon dioxide uptake.
- Rinse electrode with distilled water before and after each use.
- Gently blot electrode with lint-free wipes after each rinsing.
- See Procedure Note #1.
- Place electrode into initial calibration buffer; follow manufacturer’s instructions for measuring and reading pH level.
- Repeat with second and/or third calibration buffer measurements.
- Verify and document calibration of pH standards.
- Proceed with pH reading of samples once calibration is completed.
- Rinse and store electrode after completion of sample pH readings.
- Discard calibration buffers after use.
- Only lint-free wipes such as Kimwipes® should be used to blot the electrode. The use of cloth or paper towels to blot/wipe the electrode may produce a static charge and interfere with pH values.
- Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 78-79.
- Ruff, Bess. “How to Calibrate and Use a PH Meter: 12 Steps (with Pictures).” WikiHow, September 12, 2021.
- Modifications developed by Newcomer Supply Laboratory.