S-Mounting Medium
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- Compatible with Xylene & aliphatic hydrocarbon xylene substitutes (e.g. SlideBrite)
- Dries clear and fast
- 4oz. plastic dispensing bottle
- Incompatible with film coverslips
- Manufactured by Azer Scientific
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PRODUCT
4 oz Bottle | |
S-Mounting Medium | Part 6751 |
APPLICATION:
Newcomer Supply S-Mounting Medium is a ready-to-use permanent coverslipping medium designed for use with Slide Brite™ Xylene Substitute, other aliphatic hydrocarbon xylene substitutes, and is also compatible with xylene. Features of S-Mounting Medium include:
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- Quickly dries to a clear solid plastic.
- Does not bleach or bleed stains.
- Does not yellow or become brittle and crack over time.
- Quality of stained coverslipped sections remain unchanged indefinitely.
- Provides excellent optical quality.
METHOD:
Technique: Paraffin or frozen sections
PROCEDURE:
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- Test S-Mounting Medium compatibility with clearing agent prior to use.
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- See Procedure Note #1.
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- Complete staining procedure; dehydrate, clear with Slide Brite™, xylene or other compatible clearing agent and blot excess clearing agent from slide edges.
- Apply one to two drops of S-Mounting Medium to flow over and fully cover the tissue section(s).
- Install coverslip, one edge first, allowing any air bubbles to escape as the opposite edge is lowered to the slide.
- Blot excess S-Mounting Medium from slide.
- Dry slide in a horizontal position.
- Allow to set at room temperature for approximately 3-5 minutes.
- Dry slides a minimum of 24 hours before filing or shipping.
- To remove/replace a coverslip; completely immerse slide(s) in a compatible clearing agent. (Note: Slide Brite™ will not remove adhered coverslips as well as xylene).
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- After coverslip detaches, agitate slide in clearing solution for 1-2 minutes to remove excess mounting medium.
- Clear slide in a fresh solution of Slide Brite™, xylene or other compatible clearing agent.
- Remount with S-Mounting Medium and new coverslip.
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- Test S-Mounting Medium compatibility with clearing agent prior to use.
PROCEDURE NOTES:
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- On a glass slide mix several drops of S-Mounting Medium with an equal amount of clearing agent. If mounting medium displays separation or is not readily miscible, it is incompatible with that clearing agent.
- When not in use keep S-Mounting Medium tightly capped to maintain solution viscosity and avoid evaporation.
REFERENCES:
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- Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 128-131.
- Ravikumar, Shamala, R. Surekha, and Rooban Thavarajah. “Mounting Media: An Overview.” Journal of Dr. NTR University of Health Sciences5 (2014): 1-8.
- Modifications developed by Newcomer Supply Laboratory.