Haupt Gelatin Adhesive
SOLUTION:
500 ml | |
Haupt Gelatin Adhesive | Part 1151A |
For storage requirements and expiration date refer to individual bottle label.
APPLICATION:
Newcomer Supply Haupt Gelatin Adhesive is a blended solution of high quality gelatin, glycerin and phenol with a wide variety of procedural uses including subbed slide/direct slide coating applications. Haupt Gelatin Adhesive works to create a strong adhesive bond between tissue sections and microscopic slides to prevent or reduce the loss of sections due to the nature of the tissue and tissue treatments, such as:
- Thick sections
- Harsh staining procedures
- Animal tissues
- Plant preparations
- Bone specimens
- Resin, plastic/methyl methacrylate (MMA) sections
METHOD:
Technique: Frozen, paraffin or resin, plastic/MMA sections
Solutions: All solutions are manufactured by Newcomer Supply, Inc.
PROCEDURES:
Haupt Gelatin Adhesive Subbed Slide Preparation:
- Use only clean and dry microscopic slides.
-
Place a large drop of Haupt Gelatin Adhesive on slides, spread evenly over surface creating a thin film.
- Allow a minimum of 30 minutes drying time.
- Background staining may occur with thicker films.
- See Procedure Notes #1 and #2.
Water Bath Method for Sections on Subbed Slides:
-
Fill water bath/floatation bath with distilled or deionized water with temperature set and maintained at 5°C-10°C below the melting point of embedding medium or according to laboratory protocol.
- See Procedure Note #3.
- Float paraffin tissue sections onto Haupt subbed slides.
- Warm slide slightly on top edge of water bath to straighten section.
- Drain and dry per laboratory protocol.
Vapor Fixation Method for Sections on Subbed Slides:
- Mount paraffin or frozen sections on Haupt subbed slides; dry 1 minute.
- Under fume hood, add 2-4 ml of concentrated formaldehyde to the bottom of a Coplin jar.
-
Place slides in Coplin jar; formaldehyde should not be in direct contact with tissue sections.
- See Procedure Note #4.
- Tightly cover and place in 60°C oven for 30 minutes to 1 hour.
- Remove slides from Coplin jar; dry according to laboratory protocol.
Method for Resin, Plastic/MMA Sections on Subbed Slides:
- Place droplets of filtered/processed water on Haupt subbed slides.
- Transfer resin or plastic/MMA sections to water droplets.
- Dry, press and/or proceed according to laboratory protocol.
Method for Resin, Plastic/MMA Sections on Non-Subbed Slides:
- Place droplets of Haupt Gelatin Adhesive on clean, dry slides.
- Transfer resin or plastic/MMA sections directly to Haupt droplets.
- Add 1-2 drops of 50% ethanol atop sections.
- Manipulate sections if necessary, for up to 5 minutes, by teasing or stretching to remove any wrinkles/folds.
- Dry, press and/or proceed according to laboratory protocol.
PROCEDURE NOTES:
- Dry slides in as “dust-free” an environment as possible.
-
Store dried subbed slides indefinitely in a clean slide box at room temperature in a humidity/temperature controlled environment.
- If slides are not thoroughly dried before storing they will adhere together.
- Thoroughly clean interior/exterior of water bath/floatation bath on a daily basis to deter contaminates and the possibility of any residual adhesive build-up.
- Formalin vapor renders gelatin insoluble, affixing tissue sections to subbed slides.
REFERENCES:
- Haupt, Arthur W. "A Gelatin Fixative for Paraffin Sections." Stain Technology 5.3 (1930): 97-98.
- Huang, Bing Quan., Michael John. Sumner, Claudio Stasolla, and Edward C. Yeung. Plant Microtechniques and Protocols. Springer, 2015. 88, 94.
- Luna, Lee G. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Gaitheresburg, MD: American Histolabs, 1992. 581.
- Presnell, Janice, Martin Schreibman, and Gretchen Humason. Humason's Animal Tissue Techniques. 5th ed. Baltimore: Johns Hopkins University Press, 1997. 468.
- Skinner, Robert A. "Practical Approaches to Processing Bone: A Clinical/Research Comparative Overview." Lecture, Annual NSH Symposium/Convention, Cincinnati, Ohio. September 20, 2011.
- Modifications developed by Newcomer Supply Laboratory.