Validation Stain: Hall’s Method
Tissue: Positive staining liver.
Fixation: Formalin 10%, Phosphate Buffered (Part 1090).
Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.
Quality Control Stain: Bile Stain, Hall’s Method quality control stained slide(s) included.
Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity.
Storage: 15-30°C in a light deprived and humidity controlled environment.
Intended Use: To verify histological techniques and reagent reactivity.
Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.
CONTROL SLIDE VALIDATION:
|With Bile Stain, Hall’s Method:||Individual Stain Solution|
|Fouchet Reagent||Part 1095|
|Van Gieson Stain||Part 1404|
Newcomer Supply Bile Control Slides are for the positive histochemical staining of bile (bilirubin) substances in tissue sections and to distinguish bile pigments from other tissue pigments.
- Heat dry sections in oven according to your laboratory protocol.
- Filter Fouchet Reagent with high quality filter paper prior to use.
NEWCOMER SUPPLY VALIDATION PROCEDURE:
- Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each. Wash well with distilled water.
- See Procedure Notes #1 and #2.
- Place slides in freshly filtered Fouchet Reagent for 5 minutes.
- Wash in three changes of tap water; rinse in distilled water.
- Stain sections in Van Gieson Stain for 5 minutes.
- Rinse slides quickly in 95% ethyl alcohol.
- Dehydrate in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium.
|Bile||Emerald green to olive drab|
|Connective tissue||Pink to red|
- Drain staining slides after each step to prevent solution carry over.
- Do not allow sections to dry out at any point during procedure.
- If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.
- Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 268-269.
- Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 219.
- Modifications developed by Newcomer Supply Laboratory.