Bile

Controls Slides Histopathology Bile

Validation Stain: Hall's Method

 

Tissue: 
(+) Liver
Adhesive or Charged Slides: 
Superfrost +

Product Options:

Part # 4060A10/Set $52.00
Part # 4060B98/Set $365.00

PRODUCT SPECIFICATIONS:

Tissue:  Positive staining liver.

Fixation: Formalin 10%, Phosphate Buffered (Part 1090).

Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.

Quality Control Stain:  Bile Stain, Hall’s Method quality control stained slide(s) included.

Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity. 

Storage: 15-30°C in a light deprived and humidity controlled environment.

Intended Use: To verify histological techniques and reagent reactivity.

Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.

 

CONTROL SLIDE VALIDATION:

With Bile Stain, Hall’s Method: Individual Stain Solution  
Fouchet Reagent Part 1095
Van Gieson Stain Part 1404

   

APPLICATION:                    

Newcomer Supply Bile Control Slides are for the positive histochemical staining of bile (bilirubin) substances in tissue sections and to distinguish bile pigments from other tissue pigments.

 

PRESTAINING PREPARATION:

  1. Heat dry sections in oven according to your laboratory protocol.
  2. Filter Fouchet Reagent with high quality filter paper prior to use.

 

NEWCOMER SUPPLY VALIDATION PROCEDURE:

  1. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each.  Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
    1. See Procedure Notes #1 and #2.
  2. Place slides in freshly filtered Fouchet Reagent for 5 minutes.
  3. Wash in three changes of tap water; rinse in distilled water.
  4. Stain sections in Van Gieson Stain for 5 minutes.
  5. Rinse slides quickly in 95% ethyl alcohol.
  6. Dehydrate in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium. 

 

RESULTS:

Bile Emerald green to olive drab
Connective tissue Pink to red
Background Yellow

 

PROCEDURE NOTES:

  1. Drain staining slides after each step to prevent solution carry over.
  2. Do not allow sections to dry out at any point during procedure.
  3. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.

 

REFERENCES:

  1. Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 268-269.
  2. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 219.
  3. Modifications developed by Newcomer Supply Laboratory.