Hematoxylin Stain, Mayer Modified

Good news. We eliminated the Chloral Hydrate! Excellent for IHC’s.

Product Options:

Part # 1202A500 ml $34.40
Part # 1202B1 L $54.10
Part # 1202C1 gal $151.00

 

 

STAIN SOLUTION:                                                                                                                                               

  Part 1202A Part 1202B Part 1202C
Hematoxylin Stain, Mayer Modified 500ml 1 Liter 1 Gallon

 

Additionally Needed:  

Hematoxylin and Eosin (H&E) Control Slides Part 4278
Xylene, ACS Part 1445
Alcohol, Ethyl Denatured, 100% Part 10841
Alcohol, Ethyl Denatured, 95% Part 10842

Lithium Carbonate, Saturated Aqueous

                       OR

Scott Tap Water Substitute

Part 12215

 

Part 1380

Alcohol, Ethyl Denatured, 70% Part 10844

Eosin Y Working Solution

                      OR

Eosin-Phloxine Stain Set

Part 1072

 

Part 1082

For storage requirements and expiration date refer to individual product labels.

 

APPLICATION:

Newcomer Supply Hematoxylin Stain, Mayer Modified is a ready to use progressive hematoxylin that does not require filtering and does not contain chloral hydrate or alcohol. Due to the progressive staining nature of Mayer hematoxylin, over-staining is less likely and an acid alcohol differentiation step is not required in the staining process.  Mayer hematoxylin is an excellent choice as a counterstain in immunohistochemistry (IHC), amyloid and copper staining procedures.

The routine hematoxylin and eosin (H&E) stain is used for screening specimens in anatomic pathology, as well as for research, smears, touch preps and other applications.  Its two primary coloring agents stain all cellular material including nuclei (blue), and cytoplasmic elements (pink-red).  Popularity of this stain is due, in large measure, to its simplicity, ability to clearly demonstrate a wide variety of different tissue components, dependability, repeatability, and speed of use.

Quality Control: Since hematoxylin and eosin staining is the foundation of the diagnostic process, maintaining quality is of critical importance. Change staining solutions on a regular basis according to laboratory protocol.  Procedures will vary between laboratories depending upon volume of slides, automation vs manual staining, chemical hygiene and solution integrity.  The longevity of hematoxylin depends upon these factors and stain quality should be regularly screened with the use of an H&E control slide.

 

METHOD:

Fixation:  Formalin 10%, Phosphate Buffered (Part 1090)

Technique:  Paraffin sections cut at 5 microns

Solutions:  All solutions are manufactured by Newcomer Supply, Inc.

 

H&E STAINING PROCEDURE WITH MAYER MODIFIED:

  1. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each.  Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
  1. See Procedure Notes #1 and #2.
  1. Stain with Hematoxylin Stain, Mayer Modified, 10 to 20 minutes, depending on preference of nuclear stain intensity.
    1. See Procedure Note #3.
  2. Wash well in tap water for 3 minutes.
  3. Blue slides in Lithium Carbonate, Saturated Aqueous (Part 12215) or Scott Tap Water Substitute (Part 1380) for 10 dips.
  4. Wash in three changes of tap water; rinse in distilled water.
  5. Drain excess water from rack/slides; proceed to 70% alcohol for 10 dips.
  1. Counterstain in Eosin Y Working Solution (Part 1072) or prepared Eosin-Phloxine Working Solution (Part 1082) for 30 seconds to 3 minutes, depending on preference of intensity.
  2. Dehydrate in two changes of 95% ethyl alcohol for 1 minute each and two changes of 100% ethyl alcohol, 10 dips each.   Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium. 

 

RESULTS:

Nuclei Blue
Erythrocytes and eosinophilic granules Bright pink to red
Cytoplasm and other tissue elements Various shades of pink

 

PROCEDURE NOTES:

  1. Drain staining rack/slides after each step to prevent solution carry over.
  2. Do not allow sections to dry out at any point during staining procedure.
  3. Used as a special stain or IHC nuclear counterstain may require less time.
  4. To minimize oxidation and extend shelf life, store hematoxylin at room temperature in tightly capped container away from direct light.
  5. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.

 

REFERENCES:

  1. Bancroft, John D., and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 123.
  2. Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 114, 117.
  3. Luna, Lee G. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd ed. New York: Blakiston Division, McGraw-Hill, 1968. 36-38.
  4. Modifications developed by Newcomer Supply Laboratory