Bile

Controls Slides Histopathology Bile

Validation Stain: Hall's Method

 

Tissue: 
(+) Liver
Adhesive or Charged Slides: 
Superfrost +

Product Options:

Part # 4060A10/Set $49.00
Part # 4060B98/Set $346.00

PRODUCT SPECIFICATIONS:

Tissue:  Positive staining liver.

Fixation: Formalin 10%, Phosphate Buffered (Part 1090).

Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.

Quality Control Stain:  Bile Stain, Hall’s Method quality control stained slide(s) included.

Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity. 

Storage: 15-30°C in a light deprived and humidity controlled environment.

Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.

 

PRODUCT DESCRIPTION:

The enclosed positive control slides are intended to verify histological techniques and reagent reactivity. The intended use is for the qualitative purpose of determining positive or negative results, and not intended for any quantitative purpose. These positive control slides are produced from human surgical or autopsy tissues under carefully controlled conditions.  Quality control measures are used to deliver control slides that are as consistent as possible. 

 

CONTROL SLIDE VALIDATION:

With Bile Stain, Hall’s Method: Individual Stain Solution  
Fouchet Reagent Part 1095
Van Gieson Stain Part 1404

   

APPLICATION:                    

Newcomer Supply Bile Control Slides are for the positive histochemical staining of bile (bilirubin) substances in tissue sections and to distinguish bile pigments from other tissue pigments.

 

PRESTAINING PREPARATION:

  1. Heat dry sections in oven according to your laboratory protocol.
  2. Filter Fouchet Reagent with high quality filter paper prior to use.

 

NEWCOMER SUPPLY VALIDATION PROCEDURE:

  1. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each.  Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
    1. See Procedure Notes #1 and #2.
  2. Place slides in freshly filtered Fouchet Reagent for 5 minutes.
  3. Wash in three changes of tap water; rinse in distilled water.
  4. Stain sections in Van Gieson Stain for 5 minutes.
  5. Rinse slides quickly in 95% ethyl alcohol.
  6. Dehydrate in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium. 

 

RESULTS:

Bile Emerald green to olive drab
Connective tissue Pink to red
Background Yellow

 

PROCEDURE NOTES:

  1. Drain staining slides after each step to prevent solution carry over.
  2. Do not allow sections to dry out at any point during procedure.
  3. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.

 

REFERENCES:

  1. Carson, Freida L., and Christa Hladik. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago, Ill.: American Society of Clinical Pathologists, 2009. 268-269.
  2. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 219.
  3. Modifications developed by Newcomer Supply Laboratory.