Alcian Blue/PAS

Controls Slides Histopathology Alcian Blue / PAS

Validation Stain: Alcian Blue/PAS

 

Tissue: 
(+) Small Intestine
Adhesive or Charged Slides: 
Superfrost +

Product Options:

Part # 4022A10/Set $49.00
Part # 4022B98/Set $346.00

PRODUCT SPECIFICATIONS:

Tissue:  Positive staining small intestine.

Fixation: Formalin 10%, Phosphate Buffered (Part 1090).

Section/Glass: Paraffin sections cut at 4 microns on Superfrost™ Plus slides.

Quality Control Stain:  Alcian Blue/PAS quality control stained slide(s) included.

Reactivity: Guaranteed product specific reactivity for one year from date of receipt. Revalidate after one year to verify continued reactivity. 

Storage: 15-30°C in a light deprived and humidity controlled environment.

Before using unstained control slides, review the enclosed stained slide(s) to ensure that this tissue source is acceptable for testing needs.

 

PRODUCT DESCRIPTION:

The enclosed positive control slides are intended to verify histological techniques and reagent reactivity. The intended use is for the qualitative purpose of determining positive or negative results, and not intended for any quantitative purpose. These positive control slides are produced from human surgical or autopsy tissues under carefully controlled conditions.  Quality control measures are used to deliver control slides that are as consistent as possible. 

 

CONTROL SLIDE VALIDATION:

With Alcian Blue/PAS Stain Kit:  Part 91022A/B Individual Stain Solution
Solution A: Acetic Acid 3%, Aqueous 250/500 ml Part 10017
Solution B: Alcian Blue Stain 1%, pH 2.5 Aqueous 250/500 ml Part 1003
Solution C: Periodic Acid 0.5%, Aqueous 250/500 ml Part 13308
Solution D: Schiff Reagent, McManus 250/500 ml Part 1371
Solution E: Hematoxylin Stain, Mayer Modified 250/500 ml Part 1202

 

APPLICATION:

Newcomer Supply Alcian Blue/PAS Control Slides are for the positive histochemical staining and differentiation of acidic epithelial mucins (sialomucin, sulfomucin), stromal (mesenchymal) mucin, neutral mucins and glycogen.

                               

NEWCOMER SUPPLY VALIDATION PROCEDURE:

  1. Heat dry sections in oven according to your laboratory protocol.
  2. Deparaffinize sections thoroughly in three changes of xylene, 3 minutes each.  Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
    1. See Procedure Notes #1 and #2.
  3. Place slides in Solution A: Acetic Acid 3%, Aqueous for 3 minutes.
  4. Place slides directly into Solution B: Alcian Blue Stain 1%, pH 2.5 Aqueous for 15 minutes.
  5. Wash slides in gently running tap water for 1-2 minutes; rinse in distilled water.
  6. Place in Solution C: Periodic Acid 0.5%, Aqueous for 5 minutes.
  7. Wash in running tap water for 1-2 minutes; rinse in distilled water.
  8. Place in Solution D: Schiff Reagent, McManus for 10 minutes.
  9. Wash in lukewarm tap water for 5-10 minutes.
  10. Stain lightly in Solution E: Hematoxylin Stain, Mayer Modified for 1 minute.
  11. Rinse in running tap water for 1-2 minutes.
  12. Dehydrate in two changes each of 95% and 100% ethyl alcohol. Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium. 

 

RESULTS:

Acid epithelial mucins Violet
Neutral epithelial mucin Magenta
Glycogen Magenta
Stromal (mesenchymal) mucin Blue
Nuclei Pale blue

 

PROCEDURE NOTES:

  1. Drain slides after each step to prevent solution carry over.
  2. Do not allow sections to dry out at any point during procedure.
  3. If using a xylene substitute, closely follow the manufacturer’s recommendations for deparaffinization and clearing steps.

 

REFERENCES:

  1. Bancroft, John D., and Marilyn Gamble. Theory and Practice of Histological Techniques. 6th ed. Oxford: Churchill Livingstone Elsevier, 2008. 173-174.
  2. Carson, Freida L., and Christa Hladik Cappellano. Histotechnology: A Self-instructional Text. 4th ed. Chicago: ASCP Press, 2015. 150-151
  3. Modifications developed by Newcomer Supply Laboratory.